Giemsa stain


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stain

 [stān]
1. a substance used to impart color to tissues or cells, to facilitate microscopic study and identification.
2. an area of discoloration of the skin.
acid-fast stain a staining procedure for demonstrating acid-fast microorganisms.
differential stain one that facilitates differentiation of various elements in a specimen.
endogenous stain an intrinsic stain acquired during tooth development.
exogenous stain an intrinsic stain acquired after a tooth has erupted.
extrinsic stain a stain that can be removed from a tooth surface by polishing.
Giemsa stain a solution containing azure II-eosin, azure II-glycerin, and methanol; used for staining protozoan parasites such as Plasmodium and Trypanosoma, for Chlamydia, for differential staining of blood smears, and for viral inclusion bodies. Stained elements appear pink to purple to blue.
Gram stain a staining procedure in which bacteria are stained with crystal violet, treated with strong iodine solution, decolorized with ethanol or ethanol-acetone, and counterstained with a contrasting dye; those retaining the stain are called gram-positive, and those losing the stain but staining with the counterstain are called gram-negative.
hematoxylin and eosin stain a mixture of hematoxylin in distilled water and aqueous eosin solution, employed universally for routine examination of tissues.
intrinsic stain a stain that is within the enamel of a tooth and cannot be removed by polishing.
metachromatic stain one that produces in certain elements a color different from that of the stain itself.
nuclear stain one that selectively stains cell nuclei, generally a basic stain.
port-wine stain a persistent dark red to purple nevus flammeus that grows proportionately with the affected child and is usually found on the face. Initially it is macular, but the surface may develop angiomatous overgrowths with time. Port-wine stains often occur in association with other congenital abnormalities.
supravital stain a stain introduced in living tissue or cells that have been removed from the body.
tumor stain an area of increased density in a radiograph, due to collection of contrast material in distorted and abnormal vessels, prominent in the capillary and venous phases of arteriography, and presumed to indicate neoplasm.
vital stain a stain introduced into the living organism, and taken up selectively by various tissue or cellular elements.
Wright's stain a mixture of eosin and methylene blue, used for demonstrating blood cells and malarial parasites.

Giem·sa stain

(gēm'să),
compound of methylene blue-eosin and methylene blue used for demonstrating Negri bodies, Tunga species, spirochetes and protozoans, and differential staining of blood smears; also used for chromosomes, sometimes after hydrolyzing the cytologic preparation in hot hydrochloric acid, and for showing chromosome G bands; often used in glycerol-methanol buffer solution.

Giem·sa stain

(gēm'să stān)
Compound of methylene blue-eosin and methylene blue used for demonstrating Negri bodies, Tunga species, spirochetes and protozoans, and differential staining of blood smears; also used for chromosomes, sometimes after hydrolyzing the cytologic preparation in hot hydrochloric acid, and for showing chromosome G bands.

Giemsa,

Gustav, German bacteriologist, 1867-1948.
Giemsa chromosome banding stain - a unique chromosome staining technique used in human cytogenetics to identify individual chromosomes. Synonym(s): G-banding stain
Giemsa stain - compound used for demonstrating Negri bodies, Tunga species, spirochetes and protozoans, and differential staining of blood smears.

Giemsa stain

a solution containing azure II-eosin, azure II, glycerin and methanol; used for staining protozoan parasites, Leptospira, Borrelia, viral inclusion bodies and Rickettsia.
References in periodicals archive ?
Giemsa stain accumulated in spermatozoa with an intact acrosome (staining the acrosome region in purple).
Slides were air dried, fixed with methanol and stained with May- Grunwald Giemsa stain (Merck, Germany) according to Mohr (1981).
Parasitaemia was determined using blood smears stained with Giemsa stain from mice (Fig.
The mast cells are CD117 positive9 and the granules take up toluidine blue and Giemsa stain.
Peripheral blood smear was stained with Giemsa stain and the parasitic count was 8000/[micro]l.
One is the Giemsa stain or one of its modifications, which is applied on air dried smears and other is the pap/H&E stains which are applied on wet fixed smears.
In the laboratory, a Giemsa stain of a smear prepared from the patient's blood showed activated lymphocytes.
Basically, this consists in appropriate fixation for 24 hours, microdissection under stereomicroscope for removal of the mucosa and submucosa; evidencing of theneurons with Giemsa stain followed by dehydration and diaphanization and mounting of the whole-mounts between slide and coverslip with Permount synthetic resin.
Direct, air-dried smears are prepared and stained with a modified Giemsa stain, and results are usually issued within 24 hours.
Fresh Giemsa stain was prepared as used for staining blood smears (Kayser et al.
As a loss of substrate adherence is an early event in apoptosis, we examined morphological alterations by Giemsa stain and internucleosomal DNA fragmentation by agarose gel electrophoresis.