Giemsa stain


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stain

 [stān]
1. a substance used to impart color to tissues or cells, to facilitate microscopic study and identification.
2. an area of discoloration of the skin.
acid-fast stain a staining procedure for demonstrating acid-fast microorganisms.
differential stain one that facilitates differentiation of various elements in a specimen.
endogenous stain an intrinsic stain acquired during tooth development.
exogenous stain an intrinsic stain acquired after a tooth has erupted.
extrinsic stain a stain that can be removed from a tooth surface by polishing.
Giemsa stain a solution containing azure II-eosin, azure II-glycerin, and methanol; used for staining protozoan parasites such as Plasmodium and Trypanosoma, for Chlamydia, for differential staining of blood smears, and for viral inclusion bodies. Stained elements appear pink to purple to blue.
Gram stain a staining procedure in which bacteria are stained with crystal violet, treated with strong iodine solution, decolorized with ethanol or ethanol-acetone, and counterstained with a contrasting dye; those retaining the stain are called gram-positive, and those losing the stain but staining with the counterstain are called gram-negative.
hematoxylin and eosin stain a mixture of hematoxylin in distilled water and aqueous eosin solution, employed universally for routine examination of tissues.
intrinsic stain a stain that is within the enamel of a tooth and cannot be removed by polishing.
metachromatic stain one that produces in certain elements a color different from that of the stain itself.
nuclear stain one that selectively stains cell nuclei, generally a basic stain.
port-wine stain a persistent dark red to purple nevus flammeus that grows proportionately with the affected child and is usually found on the face. Initially it is macular, but the surface may develop angiomatous overgrowths with time. Port-wine stains often occur in association with other congenital abnormalities.
supravital stain a stain introduced in living tissue or cells that have been removed from the body.
tumor stain an area of increased density in a radiograph, due to collection of contrast material in distorted and abnormal vessels, prominent in the capillary and venous phases of arteriography, and presumed to indicate neoplasm.
vital stain a stain introduced into the living organism, and taken up selectively by various tissue or cellular elements.
Wright's stain a mixture of eosin and methylene blue, used for demonstrating blood cells and malarial parasites.

Giem·sa stain

(gēm'să),
compound of methylene blue-eosin and methylene blue used for demonstrating Negri bodies, Tunga species, spirochetes and protozoans, and differential staining of blood smears; also used for chromosomes, sometimes after hydrolyzing the cytologic preparation in hot hydrochloric acid, and for showing chromosome G bands; often used in glycerol-methanol buffer solution.

Giem·sa stain

(gēm'să stān)
Compound of methylene blue-eosin and methylene blue used for demonstrating Negri bodies, Tunga species, spirochetes and protozoans, and differential staining of blood smears; also used for chromosomes, sometimes after hydrolyzing the cytologic preparation in hot hydrochloric acid, and for showing chromosome G bands.

Giemsa,

Gustav, German bacteriologist, 1867-1948.
Giemsa chromosome banding stain - a unique chromosome staining technique used in human cytogenetics to identify individual chromosomes. Synonym(s): G-banding stain
Giemsa stain - compound used for demonstrating Negri bodies, Tunga species, spirochetes and protozoans, and differential staining of blood smears.
References in periodicals archive ?
donovani parasite was respond to food colorings and stained well in comparison with giemsa stain as control stain depending for parasite staining.
An additional Giemsa stained fecal smear confirmed the presence of the unidentified drop-shaped elements (DSE) together with G.
Further, the slides were stained with Wright's giemsa stain and then subjected to microscopic observation.
After treatment with HCl, the slides were rinsed in running tap water for 5 s and dried before staining with the aceto-orcein and Giemsa stains, as described above.
Schachner recommended Gram stains, potassium hydroxide testing, and Giemsa stains whenever a neonate has pustules.
within the red blood cells and these were of lightly basophilic pyriform structures with indistinct internal structures in Giemsa stain. Identifying parasites through blood smear evaluation was very difficult because of small size of organism and relatively low levels of parasitemia.
There were significant differences in concordance rate observed between modified Giemsa stains and each of the other preparation types in pericardial fluid slides (all P < .001).
Grunwald and Giemsa stain. They were observed for Nuclear abnormalities like Karyolysis (KL), Karyorrhexis (KR), Binucleated egg (BN) under bright field Nikon microscope under 10 x 100 magnifications.
Cells were fixed in pure methanol for 10 minutes and then immersed in a freshly prepared working Giemsa stain solution (KeyGEN Biotech, Jiangsu, China) for 20 minutes, flushed with tap water, and left to dry last.
For highlighting the presence of H.Pylori, modified Giemsa stain was done on one section including antral and corporal mucosa6.
Tzanck smear was taken from each patient at presentation and stained by Giemsa stain for giant cells and evaluated by histopathologist.