gene cloning

gene cloning

The identification and isolation of a gene and the production of a number of identical copies of it.

gene cloning

the technique of genetic engineering in which specific genes are excised from host DNA, inserted into a VECTOR (2) and introduced into a host cell, which then divides to produce many copies (clones) of the transferred gene.
References in periodicals archive ?
Advances over the last decade have obviated the need for traditional gene cloning, meaning that almost any DNA sequence, natural or synthetic, can be chemically synthesised and assembled quickly.
Other aspects included primer design, polymerase chain reaction; gene cloning in bacterial expression systems; cell culture techniques; cell transfection; western blotting and confocal microscopy.
In plants functional genomic studies, gene cloning and vector construction for transformation are common procedures.
Modern biotechnology involves the more precise ability to effect genetic changes to produce improved or even new traits and includes such new techniques as recombinant DNA and gene cloning collectively referred to as 'genetic engineering'.
'The leading organization on cloning was categorical in stating that 'Gene cloning is the most common type of cloning done by researchers at the National Human Genome Research Institute (NHGRI).
calidifontis, by using gene cloning in pET101 cloning vector and purification of APU was achieved with immobilized metal affinity chromatography technique.
Gene cloning: PCR products were cloned into 2886 base pair (bp) pTZ57R/T vector by using InsTAclone PCR cloning kit (Thermo Scientific).
The company utilizes a proprietary microwell array technology to identify cell type and measure functioning of individual cells from tissue samples, and to retrieve individual live cells of interest, for cell culture or gene cloning.
A Novel Lipase from Pseudomonas fluorescens HU380/: Gene cloning, overproduction , renaturation-activation, two-step purification and characterization, Journal of Bioscience and Bioengineering, 2003; 96 (3), 242-249.
In his inaugural address, Dr Naeem Khan said that Punjab University Centre of Excellence in Molecular Biology (CEMB) had developed laboratory scale interferon vaccine for hepatitis-C patients through pharmaceutical protein gene cloning. He hoped that the symposium would help create public awareness for the prevention and cure of infectious diseases like polio, pneumonia, yellow fever, measles, meningitis and bacteraemia.
Gene Cloning and DNA Analysis: An Introduction, 7th Edition