Gene Knockout Technique

An experimental technique used in genetics in which a normal gene is replaced by a defective gene either at the exact same chromosomal site—hence, the normal gene is ‘knocked out’ by the defective gene—as occurs with the yeast genome, or the DNA is inserted at random sites, as occurs in mammalian cells
References in periodicals archive ?
Effective multiple gene knockout techniques have been developed in Sulfolobus [2-4].
In addition to the widely used multiple gene knockout techniques in S.