For convenience of discussion isoform pI 4.5 and isoform pI 6.2 were designated as GST1 and GST2, respectively.
On the other hand, GST2 displayed different physical characteristic compared to GST1.
Table 1 indicates that both GST1 and GST2 had highest affinity towards ethacrynic acid compared to the other substrates.
It shows that the strain has 10 putative GSTs of which two of the expressed proteins have close characteristics to GST1 and GST2. As shown in Table 2, The UniProt identifiers of F0KLY9 (putative glutathione S-transferase) and F0KKB0 (Glutathione S-transferase III) are similar in MW and pI values to GST1 and GST2, respectively.
Isoelectric focusing determined that the isoforms, named GST1 and GST2, have pI values of 4.5 and 6.2, respectively.
Z = 4,369 - 0,925 x BPK1 - 2,6156 x GST1 + 16,8242 x TP1 + 4,5762 x IK1 + 2,6439 x GP1 + 0,9115 x SPK1 - 20,0507 x TVP/T1 + 5,3164 x NP/T1 + 39,0135 x TVP/P1 + 5,9507 x GST2
+ 7,2504 x TP2 - 16,7569 x IK2 + 18,8240 x GP2 + 7,8331 x TVP/T2 - 12,2667 x NP/T2 - 41,6082 x TVP/P2 + 1,1088 x GST3 - 26,2628 x TP3 + 8,6205 x IK3 + 54,9958 x GP3 + 18,0501 x TVP/T3 + 9,7489 x NP/T3 - 4,1720 x BP3 - 7,0197 x TVP/ P3 - 5,8053 x GP/T3.