* Provides GSH
, which supports the detoxification of xenobiotics in the liver.
[8,22] Furthermore, according to our finding, PZA diminished GSH
Similarly, the percentage of sperm with fragmented DNA (Tunel positive) in those pretreated with DTT for 30 min was greater (p<0.05) than those exposed to 2ME and GSH
for 2 hr (Figure 4).
Detection of GSH
. For fluorescent detection of GSH
, varying volumes of 10 mM GSH
solutions were mixed with the AuNCs-BSA solution containing [Cu.sup.2+] which was prepared by adding 30 [micro]L 10 mM [Cu.sup.2+] solution to 250 [micro]L AuNCs-BSA solution, and the mixtures were diluted to 5 mL with HEPES buffer (pH=7.2).
LIM was incubated in MLMs or HLMs supplemented with GSH
or NAC and NAL as trapping agents.
Herein, we present for the first time a fluorescent probe based on o-phthaldialdehyde-assisted Si[O.sub.2] particles for GSH
determination with a "turn-on" strategy.
Sin embargo, este [H.sub.2][O.sub.2] es detoxicado por las enzimas catalasa y GSH-Px, esta ultima utilizando al GSH
que se transforma a disulfuro de glutation, que posteriormente, bajo la accion de la glutation reductasa, regenera las dos moleculas de GSH
In contrast, GSH
levels were significantly lower in diabetic-, sham PEMF-, and diabetes+PEMF-treated rats than in control rats (p<0.001), whereas CAT levels were similar in the four groups.
The mean levels of MDA, GSH
, TAS, and TOS in the study groups are shown in Table 1.
On the first, third, and seventh days after CPIP injury, we evaluated the withdrawal threshold to determine the effects of GSH
. All testing was performed by a researcher blinded to the treatments.
Neuroprotection was investigated in a model of endogenous oxidative stress, where extracellular glutamate blocks the glutamate-cystine antiporter system [Xc.sup.-] leading to deprivation of cystine and its reduced form cysteine, the rate-limiting substrate for the synthesis of GSH
. The subsequent GSH
depletion leads to accumulation of reactive oxygen species and cell death by oxidative stress (recently reviewed in ).