LY6G6D binds to growth-factor-receptor-bound protein2 (Grb2) and Grb7
and activates downstream signal pathways, including the RAS/MAPK pathway .
HER2-enriched breast tumors are characterized by high expression of ERBB2 at the RNA and protein level and by increased levels of genes coamplified with ERBB2 such as typically GRB7
. Such tumors express luminal genes at an intermediate level and do not express or express at low levels basal-related genes such as KRT5.
(75) SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture)-based phosphoproteomics study showed that, in addition to the above-described SH2-containing proteins, the EPIYA-A peptide can bind to SHP1, the EPIYA-B peptide can bind to SHP1, Grb2, and SHP2, and the EPIYA-C peptide can bind to Grb2, Grb7
, SHP1, and Ras-GTPase activating protein (GAP) in a manner that is dependent on EPIYA tyrosine phosphorylation, indicating the potential of tyrosine-phosphorylated EPIYA segments in promiscuously binding with numerous SH2 domains.
The cancer genes are comprised of a proliferation set (5 genes: Ki67, STK15, Survivin, CCNB1, MYBL2), estrogen hormone receptor genes (5 genes: ER, PGR, BCL2, SCUBE2), HER2neu set (2 genes: GRB7
, HER2), invasion set (2 genes: MMP11, CTSL2), and GSTM-1 (1 gene).
It has high expression of several genes in the ERBB2 amplicon at 17q22.24, including ERBB2 and GRB7
. About 71% of the tumors in this subtype have a p53 mutation and activation of receptor tyrosine kinase pathways, such as FGFR4, EGFR, and HER2; and 39% of tumors have a PIK3CA mutation.
Os 16 genes incluem aqueles relacionados ao RE (ER, PR, BCL2 e SCUBE2), proliferacao (Ki67, STK15, Survivin, CCNB1 e MYBL2), HER2 (HER2 e GRB7
), invasao (MMP11 e CTSL2), alem de tres genes adicionais relacionados (GSTM1, CD68 e BAG1).
The growth factor receptor-bound protein 10 (Grb10) is a member of the Grb7
family adaptor molecules that includes Grb10, Grb14 and Grb7
(For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) p-value Gene Fold Gene 1 x symbol change ID [10.sup.-2] ESR2 2.00 Estrogen receptor 2 3.1 GRB7
1.99 Growth factor 2.2 receptor-bound protein 7 MAPK3 1.72 Mitogen-activated 3.2 protein kinase 3 MLHI 1.85 MutL homolog 1, 3.7 colon cancer, nonpolyposis type 2 (E.
SH2 domain containing proteins such as growth factor receptor-bound protein 2 (GRB2), growth factor receptor-bound protein 7 (GRB7
), growth factor receptor-bound protein 14 (GRB14), protein tyrosine phosphatase nonreceptor type 11 (SHP-2), and phosphoinositide-3-kinase (PI3K) is recruited and transphosphorylated by TIE2 .
Nine of the Oncotype DX gene primer sets (for BAG1, CCNB1, SCUBE2, GRB7
, ERBB2, MK167, MYBL2, PGR, and TFRC genes) span intron sequences and so do not detect genomic DNA; the 12 remaining Oncotype DX genes were tested for sensitivity to genomic DNA added into an FPET RNA pool.
Expression of HER2 and the coamplified genes GRB7
and MLN64 in human breast cancer: quantitative realtime reverse transcription-PCR as a diagnostic alternative to immunohistochemistry and fluorescence in situ hybridization.
Basal-like subtype was characterized by high expression of cytokeratins (CKs) 5 and 17, laminin, and fatty acid-binding protein 7, whereas HER2 overexpressing was characterized by high expression of several genes in the ERBB2 amplicon at 17q22.24, including ERBB2 and GRB7