Recently, GPR125 has been introduced as a marker for mouse spermatogonial stem/progenitor cells (5), (6).
In the next step, after expanding human spermatogonial stem cells, we evaluate our culture system by immunocytochemistry test to stain GPR125 protein as surface marker in human spermatogonial stem cells.
After 52 days of culture, the cells were trypsinated and cytospind onto slides for DAB staining GPR125 protein as surface marker on these cells.
The specific culture of human spermatogonial stem cells was evaluated and approved by collecting the cultured cells after 52 days and DAB staining GPR125 as specific surface marker on these cells.
Seandel et al, have identified GPR125 as a surface marker for self renewing clonogenic spermatogonial progenitor cells in mouse (6).
In this study, the culture process was evaluated after 52 days of specific human spermatogonial stem cells culture by GPR125 staining.