Caption: FIGURE 1: The change of the coefficient of variation CV for 22 proteins: APOL6, CASP1, CNP, CXCL10, DHX58, DRAP1, DYNLT1, FAM46A, GEMIN4, GORASP1, IFITM1, IRF7, PLSCR1, RTP4, SAMD9, SAMHD1, SIGLEC1, TAF1C, TLR7, TNFAIP6, TREX1, and ZBP1, The %-axis denotes the time (unit: h), It indicates that as the critical transition occurs, that is, the time evolves towards t = 45 h (see the vertical black line), the coefficient of variation CV for 22 proteins above significantly increases in the symptomatic group and has no obvious change in the asymptomatic group, Therefore, these 22 proteins can be viewed as DNBs for early-warning signals,
Caption: FIGURE 2: The change of the indicator TPD for 22 proteins: APOL6, CASP1, CNP, CXCL10, DHX58, DRAP1, DYNLT1, FAM46A, GEMIN4, GORASP1, IFITM1, IRF7, PLSCR1, RTP4, SAMD9, SAMHD1, SIGLEC1, TAF1C, TLR7, TNFAIP6, TREX1, and ZBP1.
For example, the SNPs rs274034 and rs7813 located in GEMIN4 (gem nuclear organelle associated protein 4), one of the miRNA machinery genes were found to contribute to prostate cancer protection/risk in patients carrying the GC and TT variant genotypes (76).
Genetic variants in the microRNA machinery gene GEMIN4 are associated with risk of prostate cancer: a case-control study of the Chinese Han population.
Interactions modifying BC associations were observed with SNPs in the DICER, GEMIN4, and DiGeorge critical region-8 (DGCR8) genes, and in GEMIN3 and GEMIN4, predicting diastolic and systolic BP, respectively.
We also observed 5.58-mmHg higher BP (95% CI, 3.01-8.14) among GEMIN4 rs 1062923 homozygous recessive carriers in response to a 1-SD change in BC, but only 2.87 (95% CI, 2.10-3.65) in heterozygotes and homozygous wild-type carriers.
AGO2, GEMIN3, and GEMIN4 then interact with miRNA to form a ribonucleoprotein, which guides the miRNA into the RNA-induced silencing complex (RISC), where the miRNA strand anneals to the 3' untranslated regions (UTRs) of target mRNAs, promoting translational repression or mRNA degradation.
Although BC interactions with SNPs in DGCR8, DICER, and GEMIN4 were observed in models of DBP, only interactions with single SNPs in GEMIN3 and GEMIN4 were predictive of SBP.
In our analyses, we found that BC interactions with SNPs in GEMIN4 were associated with SBP and DBP.