Sections were then incubated with the primary antibodies in dilution buffer (PBS containing 0.1% Triton X-100, 1% BSA) overnight at 4 [degrees]C as follows: 1) goat polyclonal anti-GDF9 primary antibody (Santa Cruz Biotechnology, CA, USA) at a dilution of 1:100 and 2) rabbit polyclonal anti-BMP15 (GDF9B
) primary antibody (Santa Cruz Biotechnology, CA, USA) at a dilution of 1:50.
The BMP15 protein is produced and secreted by the oocyte, also known as GDF9B
, and besides showing a significant mitogenic role over the granulosa cells, also contributes positively to follicular development during the initial and final stages of folliculogenesis (Otsuka et al., 2000; Juengel et al., 2004b; Lima et al., 2010).
BMP15 gene, also called, GDF9b
, has been mapped to the sheep X- chromosome comprising 6648 nucleotide full length coding sequence and contains two exons which are separated by an intron of about 5309 nucleotides and encodes protein of 394 amino acid (Galloway, et al., 2000).
One of the latest members of this super family is Bone Morphogenetic protein 15 (BMP15) or GDF9B
De los 35 miembros de la superfamilia del factor de crecimiento transformante-[beta], dos de ellos derivan del ovocito, el factor de crecimiento y diferenciacion 9 (GDF9) y la proteina morfogenica osea (BMP15, tambien conocida como GDF9B
), y ambos son requeridos para el crecimiento folicular (Juengel et al., 2004).
In 1998 two groups independently described the bone morphogenetic protein 15 (BMP15), also known as growth and differentiation factor 9B (GDF9B
), in the mouse ovary.