was calculated by the version 5.0.0 of the geecee program (Rice et al., 2000) in Galaxy (Blankenberg et al., 2007) for the genome and CDS of three species (https://main.g2.bx.psu.edu/).
We tested the DNA extract by using 2 panchordopoxvirus PCRs specific for the variable GC content
of the genera included in the subfamily Chordopoxvirinae and other unclassified chordopoxviruses (19).
Table 2 shows the description, sequence length, and GC content
for each isolate.
However, the TERT promoter has proved to be a challenging region to amplify due to its high GC content
(>80%), homopolymer runs, and low sequence complexity (2).
These include genes with complex sequence contexts such as pseudogenes, genetic rearrangements, and a high GC content
. Regions of high homology with other genomic regions, such as pseudogenes or gene duplication events, may lead to false-positive and/or false-negative results due to mismapped reads.
A common challenge in diagnostics is generating sufficient reads through the first exons of genes, where GC content
tends to be higher than average.
The GC content
of the host-derived sequences, in most cases, existed in about 40%, while GC content
of the symbiont was in about 54% (Fig.
Schuster, "GC content
dependency of open reading frame prediction via stop codon frequencies" Gene, vol.
The GC content
of the draft genome is 25.44%, which is almost identical to the GC content
of the recently published draft genome of Methanobrevibacter arboriphilus strain ANOR1 (25.46%) , and is in accordance with the overall trend that most Methanobrevibacter and Methanosphaera species genomes tend to have low GC contents
between ~24 and 33%, indicating that an early divergent evolution of these two genera from other Methanobacteriales groups may have occurred.
The genomic GC content
was about 40~45% in vertebrate.
Amplicons were designed to include variable characters, lengths, and GC content
. Primers for HRM were developed based on the following criteria: (i) primer pairs should yield an amplicon of <300 bp; and (ii) primer pairs should yield amplicons with a sufficient number of variable sites to enable species discrimination.