Flies crossed to GAL4
drivers crossed to UAS-RNAi lines) and controls (GAL4
drivers or UAS-RNAi crossed to w1118) were raised at 25oC until the adults emerged (F1 generation); once collected adults were raised at 29oC for the appropriate times.
EFFECTS OF HEAT SHOCK AND GAL4
ON DROSOPHILA OOGENESIS.
AD-AMOT, or Gal4
AD-CADM1 alone did not activate the reporter gene HIS3.
Full-length PB1-F2 complementary DNA (cDNA) was cloned into pGBKT7 downstream of the Gal4
gene in fusion as bait, and a pGAD-based human lymphoid cDNA library (Clontech) was used for screening AH109 yeast cells according to the manufacturer's protocol.
When transcriptional PIC was formed at 25[degrees]C, both wild-type and med6-ts2 Pol II holoenzymes showed 23- and 21-fold activation of GAL4
enhancer-containing template (GAL4
:G-) by Gal4-VP16 over basal transcription from GCN4:G-template, respectively (Figure 4, lanes 1 and 2).
Ptashne, "Specific DNA binding of GAL4
, a positive regulatory protein of yeast," Cell, vol.
The cell culture assays were performed after transfection of (i) pBIND-PPAR[beta]/[delta], expressing Gal4-DBD and PPAR[beta]/[delta]-LBD fusion protein, and (ii) pGRE-LUC, which contains a Gal4
response element followed by firefly luciferase reporter gene.
cDNAs corresponding to FULL (225 amino acids), NTR (N-terminal region of 1-118 amino acids), and CTR (C-terminal region of 119-225 amino acids) were inserted into the EcoRI and PstI sites of pGBKT7 (Clontech, USA) fusing with the GAL4
(yeast transcription activator protein) fused to the PPAR[gamma] ligand-binding domain (LBD) and a firefly luciferase construct under the control of UAS (upstream activating sequence) were used.
The IRMA network data is a subnetwork embedded in Saccharomyces cerevisiae consisting of 5 genes: CBF1, GAL4
, SWI5, GAL80, and ASH1.
An efficient binary system for gene expression in the silkworm, Bombyx mori, using GAL4