Flies crossed to GAL4
drivers crossed to UAS-RNAi lines) and controls (GAL4
drivers or UAS-RNAi crossed to w1118) were raised at 25oC until the adults emerged (F1 generation); once collected adults were raised at 29oC for the appropriate times.
EFFECTS OF HEAT SHOCK AND GAL4
ON DROSOPHILA OOGENESIS.
AD-AMOT, or Gal4
AD-CADM1 alone did not activate the reporter gene HIS3.
Full-length PB1-F2 complementary DNA (cDNA) was cloned into pGBKT7 downstream of the Gal4
gene in fusion as bait, and a pGAD-based human lymphoid cDNA library (Clontech) was used for screening AH109 yeast cells according to the manufacturer's protocol.
When transcriptional PIC was formed at 25[degrees]C, both wild-type and med6-ts2 Pol II holoenzymes showed 23- and 21-fold activation of GAL4
enhancer-containing template (GAL4
:G-) by Gal4-VP16 over basal transcription from GCN4:G-template, respectively (Figure 4, lanes 1 and 2).
Ptashne, "Specific DNA binding of GAL4
, a positive regulatory protein of yeast," Cell, vol.
The cell culture assays were performed after transfection of (i) pBIND-PPAR[beta]/[delta], expressing Gal4-DBD and PPAR[beta]/[delta]-LBD fusion protein, and (ii) pGRE-LUC, which contains a Gal4
response element followed by firefly luciferase reporter gene.
cDNAs corresponding to FULL (225 amino acids), NTR (N-terminal region of 1-118 amino acids), and CTR (C-terminal region of 119-225 amino acids) were inserted into the EcoRI and PstI sites of pGBKT7 (Clontech, USA) fusing with the GAL4
By turning up the temperature on these genetically engineered GAL4
fly lines, which were also developed at Janelia, researchers were able to study how different sections of the brain contributed to particular behaviors.
(yeast transcription activator protein) fused to the PPAR[gamma] ligand-binding domain (LBD) and a firefly luciferase construct under the control of UAS (upstream activating sequence) were used.
The IRMA network data is a subnetwork embedded in Saccharomyces cerevisiae consisting of 5 genes: CBF1, GAL4
, SWI5, GAL80, and ASH1.
An efficient binary system for gene expression in the silkworm, Bombyx mori, using GAL4