Besides primary endosymbionts, white fly harbors secondary endosymbionts namely Hamiltonella (Zchori-Fein and Brown, 2002), Rickettsia (Gottlieb et al., 2006), Cardinium (Zchori-Fein and Perlman, 2004), Fritschea (Everett et al., 2005), Arsenophonus (Baumann et al., 2004) and Wolbachia (Skaljac et al., 2010; Tram et al., 2003).
Fritschea belongs to genus Candidatus, order Chlamydiales was for the first time reported in 2003 (Thao et al., 2003) and is different from other endosymbionts in a manner that it is only documented in gut of whitefly.
It is found that Fritschea is present in all samples, Hamiltonella endosymbiont is present in Sadiqabad, Rahim Yar Khan, Bahawalpur, Multan and Khanewal sample, but it was not found in Vehari.
In Sadiqabad and Rahim Yar Khan Hamiltonella, Arsenophonus, Fritschea, Cardinium were present, in Bahawalpur Hamiltonella and Fritschea were present, in Multan Arsenophonus and Cardinium were present, in Vehari Rickettsia, Fritschea, Cardinium were found but in Khanewal Hamiltonella, Arsenophonus and Fritschea were present.
Bahawalpur which is a bridge between Multan and Rahim Yar Khan has two common endosymbionts that are Fritschea and Hamiltonella.