The AF of tissues arising from cell endogenous fluorophores
serves as a major obstacle to IF analysis.
In conclusion, we developed a turn-on fluorescent probe for Cys based on a coumarin-derived fluorophore
. The sensing mechanism involved the Cys-induced cleavage of the DNBS group and the follow-up release of the coumarin fluorophore
, which was confirmed by HPLC and spectral results.
However, an experimental validation of the suitability of these fluorophores
to retain their selectivity when used simultaneously has not been reported.
A restricted class of molecules, fluorophores
, reach the ground state by emitting the energy as light at a wavelength equal to or longer than the wavelength absorbed.
Any photon originating from a fluorophore
located in the slit image, and emitted into the angle subtended by the collection lens will arrive at the CCD detector.
may only fluoresce when excited by "invisible" UV light.
Four consecutive imaging series are performed in order to collect (1) RGB image at white LED illumination, helping to reveal hidden subcutaneous structures; (2) four spectral images at narrowband LED illumination for mapping of the main skin chromophores; (3) video-images under green LED illumination for mapping of skin blood perfusion; (4) autofluorescence video-images under UV LED irradiation for mapping of the skin fluorophores
. To improve the reliability of diagnostics, manipulation with maps of different parameters (i.e.
Additionally, filters with fixed spectral signatures severely limit the flexibility of fluorophore
excitation provided by the supercontinuum laser source.
The development of fluorescent probes that are stable and significantly brighter than traditional organic fluorophores
has been of considerable interest to realize biosensors with high sensitivity [19,20].
The original concept of this sensing scheme involves a movement of the fluorophore
toward the alkylthiol gold nanoparticles detained in the oily phase of a hydrogel/ether emulsion (1-6 [micro]m droplets) , with the ether droplets as containers for ensembles of the plasmophore complex .
Conjugating CPPs to a fluorophore
then enables improved visualization of the tumor.
The principle of operation is to trap an oxygen-sensitive fluorophore
or pH indicator dye in a sol-gel host matrix that can be applied to the tip of a fibre, an adhesive membrane such as a patch or a flat substrate such as a cuvette.