flow cytometry(redirected from Fluorescence activated cell sorter)
Also found in: Dictionary, Encyclopedia.
the counting of cells, especially blood cells.
flow cytometry a cytometric technique in which cells suspended in a fluid flow one at a time through a focus of exciting light, which is scattered in patterns characteristic to the cells and their components; cells are frequently labeled with fluorescent markers so that light is first absorbed and then emitted at altered frequencies. A sensor detecting the scattered or emitted light measures the size and molecular characteristics of individual cells; tens of thousands of cells can be examined per minute and the data gathered is processed by computer.
a method of measuring fluorescence from stained cells that are in suspension and flowing through a narrow orifice, usually in combination with one or two lasers to activate the dyes; used to measure cell size, number, viability, and nucleic acid content with the aid of acridine orange, Kasten fluorescent Feulgen stain, ethidium bromide, trypan blue, and other selected staining reagents.
Synonym(s): flow cytophotometry
Cytometry performed by suspending cells in a liquid and passing them through a light beam, often after applying fluorescent stains. The measured characteristics of the scattered light correlate with cell properties such as volume, morphology, and pigmentation.
flow cytometryLab medicine Analysis of biological material by detecting the light-absorbing or fluorescing properties of cells or subcellular fractions such as chromosomes that have been labeled with monoclonal antibodies raised against various antigens, tagged with fluorochrome markers and passed in a narrow stream through a laser beam; the cells can be separated with automated sorting devices by size, intensity and type of fluorescence, and DNA ploidy analyzed. See Cell sorting, Cf Image analysis, Laser scanning cytometry.
flow cy·tom·e·try(flō sī-tom'ĕ-trē)
A method of measuring fluorescence from stained cells that are in suspension and flowing through a narrow orifice, usually with one or two lasers to activate the dyes; used to measure cell size, number, viability, and nucleic acid content.