flow cytometry


Also found in: Dictionary, Acronyms, Encyclopedia, Wikipedia.

cytometry

 [si-tom´ĕ-tre]
the counting of cells, especially blood cells.
flow cytometry a cytometric technique in which cells suspended in a fluid flow one at a time through a focus of exciting light, which is scattered in patterns characteristic to the cells and their components; cells are frequently labeled with fluorescent markers so that light is first absorbed and then emitted at altered frequencies. A sensor detecting the scattered or emitted light measures the size and molecular characteristics of individual cells; tens of thousands of cells can be examined per minute and the data gathered is processed by computer.

flow cy·tom·e·try

a method of measuring fluorescence from stained cells that are in suspension and flowing through a narrow orifice, usually in combination with one or two lasers to activate the dyes; used to measure cell size, number, viability, and nucleic acid content with the aid of acridine orange, Kasten fluorescent Feulgen stain, ethidium bromide, trypan blue, and other selected staining reagents.
Synonym(s): flow cytophotometry

flow cytometry

n.
Cytometry performed by suspending cells in a liquid and passing them through a light beam, often after applying fluorescent stains. The measured characteristics of the scattered light correlate with cell properties such as volume, morphology, and pigmentation.

flow cytometry

Lab medicine Analysis of biological material by detecting the light-absorbing or fluorescing properties of cells or subcellular fractions such as chromosomes that have been labeled with monoclonal antibodies raised against various antigens, tagged with fluorochrome markers and passed in a narrow stream through a laser beam; the cells can be separated with automated sorting devices by size, intensity and type of fluorescence, and DNA ploidy analyzed. See Cell sorting, Cf Image analysis, Laser scanning cytometry.

flow cy·tom·e·try

(flō sī-tom'ĕ-trē)
A method of measuring fluorescence from stained cells that are in suspension and flowing through a narrow orifice, usually with one or two lasers to activate the dyes; used to measure cell size, number, viability, and nucleic acid content.

flow cytometry

A medical laboratory technique used in machines that automate the analysis of cells or cell nuclei that have been labelled with fluorescent dyes specific for certain substances. The cells are suspended in a fluid, move in a flow stream, and are illuminated by a laser beam of known wavelength. The emitted light from the fluorescent markers are detected by photomultiplier tubes. Up to six variables can be measured simultaneously.

flow cytometry

a method for sorting and identifying CELLS without culturing them. A moving fluid containing, cells, for example BACTERIA, is forced through a small opening. The cells become arranged in a line. If the fluid is then illuminated with, for example, a laser beam, the amount of light scattered can be used to determine the shape, size and density of the cells, and this is analysed by computer. Cells that are naturally fluorescent or that can be tagged with fluorescent dye can be detected by virtue of the fluorescence they produce.
References in periodicals archive ?
In the positive control, the dead cells were measured at approximately 98% by flow cytometry using PI (Figures 1a, b) and 100% by microscopic method using TB.
The bacterial suspensions were stained with NR (9.42 mM) for 5 min and analyzed by flow cytometry.
See the Global Report on Flow Cytometry at https://www.alliedmarketresearch.com/flow-cytometry-1422-market
The Diagnostic Immunology Resource Committee acts as the expert scientific and educational resource for the College of American Pathologists (CAP) in diagnostic immunology and flow cytometry. Volunteer members from 4 academic and 2 private, medium-to-large, flow cytometry laboratories oversee the proficiency testing (PT) for hundreds of flow cytometry laboratories enrolled in our surveys.
BD Life Sciences reported on Monday the formation of a strategic partnership to integrate its flow cytometry data acquisition software with FlowJo's data analysis platform for the research market.
"Flow cytometry is now an established technology, which is now widely used as standard practice for making diagnosis of hematologic malignancies.
Sysmex is conducting a clinical study assessing Merck Millipore's Amnis imaging flow cytometry technology for hematologic disorders such as leukemia.
The development of sensitive detection methods such as flow cytometry techniques has allowed identification and enumeration of intracellular particles such as proteins and nucleic acids 1'.
In most cases, researchers count CECs by fluorescence microscopy (2), analyze the cell mixture with flow cytometry (14, 15), or combine either of these with ELISA (10).
In recent years, the scope of practice for clinical laboratory scientists (CLS) has expanded to include flow cytometry. The job analysis for CLS entry level tasks completed in 2008 by the National Credentialing Agency for Laboratory Personnel (NCA) identified for the first time that CLS practitioners expect new graduates to have some knowledge and skill with flow cytometry.
Applications of flow cytometry in stem cell research and tissue regeneration.
Based in Seattle, Washington, Cytopeia develops and markets flow cytometry cell sorting instruments, including the Influx cell sorter.