Comparison of red-shifted firefly luciferase
Ppy RE9 and conventional Luc2 as bioluminescence imaging reporter genes for in vivo imaging of stem cells.
coli strain BL21 with pET16b plasmid containing firefly luciferase
was grown in LB medium and purified by Ni-Sepharose affinity chromatography, as reported earlier .
The use of firefly luciferase
reporter genes in a number of intracellular microorganisms including Mycobacterium tuberculosis (49) has facilitated antimicrobial drug testing and discovery.
Methodological aspects on the firefly luciferase
assay of adenine nucleotides in whole blood and red blood cells.
Residues of the first 60 fractions representing the more polar part of the extract were dissolved in 270 [micro]l DMEM/F-12 and 100 [micro]l per well used in a promoter reporter gene assay as described above using both murine (controlling a Renilla luciferase gene) and human (controlling a firefly luciferase
gene (Prinzen et al.
To confirm the hypothesis that miR-29 directly regulates TET2 expression, the 3'UTR of TET2 containing these sites was cloned downstream of the firefly luciferase
gene in the pMIR-REPORT vector.
values generated by the reporter plasmid were normalized to Renilla luciferase values.
To test for promoter activity, the 5'-flanking region containing promoter with and without intron 1 was fused to the firefly luciferase
gene in pGV-B2 (TOYO INK MFG, Japan) as follows.
activity was normalized to Renilla luciferase expression.
The pyrophosphates were converted by pyruvate orthophosphate dikinase into ATP, which was detected in a luminescence assay ([lambda] = 530 run) using firefly luciferase
In research, ATP, the most common organic substrate molecule in all of metabolism and found in all living cells, can be removed and assayed with firefly luciferase