luciferase

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luciferase

(lo͞o-sĭf′ə-rās′, -rāz′)
n.
An enzyme present in the cells of bioluminescent organisms that catalyzes the oxidation of luciferin and ATP, producing light.
The American Heritage® Medical Dictionary Copyright © 2007, 2004 by Houghton Mifflin Company. Published by Houghton Mifflin Company. All rights reserved.

luciferase

A mono-oxygenase enzyme (EC 1.13.12.7) that catalyses bioluminescent reactions, using luciferin as a substrate.
Segen's Medical Dictionary. © 2012 Farlex, Inc. All rights reserved.

luciferase

an oxidizing enzyme that, acting on the bioluminescent substance luciferin, causes luminosity in glow-worms and other insects. see LUMINESCENCE.
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005
References in periodicals archive ?
The pHIV plasmid is a lentiviral construct for bicistronic expression of the codon-optimised firefly luciferase (luc2) and ZsGreen (via an IRES link) under the constitutive EF1[alpha] promoter.
This cell line was further transduced with lentivirus to stably express either eGFP [20] and firefly luciferase [34] and used to track stem cell distribution in vivo.
This assay is based on the transient transfection of the 293T cell line with three plasmids: pBIND-PPAR[beta]/[delta] encoding a chimera of Gal4-DBD and PPAR[beta]/[delta]-LBD genes (PPAR/GAL4); pGRE-LUC, which owns one GAL4 response element upstream of a firefly luciferase reporter gene ([LUC.sub.F]); and the pRL vector, which constitutively express Renilla luciferase ([LUC.sub.R]).
Borghei and Hall (2014) used firefly luciferase to develop a red-shifted ATP sensor.
MAbs recognizing FFL(Firefly luciferase) specificly were developed by obtaining hybridoma cell lines from the immunized animals injected with the firefly luciferase protein .
The luciferase activities were evaluated through Dual Luciferase Reporter Assay System (Promega) which was normalized by dividing firefly luciferase activity with that of Renilla luciferase activity.
Many yeast based detection systems involve either the colorimetric detection [24, 29] or the firefly luciferase reporter [29].
The PCR product was inserted into the pGL3-Basic Vector (Promega), which contained a firefly luciferase gene.
Their work, "Designing Quantum Rods for Optimized Energy Transfer with Firefly Luciferase Enzymes," was published online May 23 in Nano Letters and is forthcoming in print.
The scientists combined a protein obtained from firefly luciferase with a special dye that allows the protein to emit near-infrared light.
The use of firefly luciferase reporter genes in a number of intracellular microorganisms including Mycobacterium tuberculosis (49) has facilitated antimicrobial drug testing and discovery.