The pHIV plasmid is a lentiviral construct for bicistronic expression of the codon-optimised firefly luciferase
(luc2) and ZsGreen (via an IRES link) under the constitutive EF1[alpha] promoter.
This cell line was further transduced with lentivirus to stably express either eGFP  and firefly luciferase
 and used to track stem cell distribution in vivo.
This assay is based on the transient transfection of the 293T cell line with three plasmids: pBIND-PPAR[beta]/[delta] encoding a chimera of Gal4-DBD and PPAR[beta]/[delta]-LBD genes (PPAR/GAL4); pGRE-LUC, which owns one GAL4 response element upstream of a firefly luciferase
reporter gene ([LUC.sub.F]); and the pRL vector, which constitutively express Renilla luciferase ([LUC.sub.R]).
Borghei and Hall (2014) used firefly luciferase
to develop a red-shifted ATP sensor.
The firefly luciferase
activities of test plasmids were normalized to renilla luciferase activities.
MAbs recognizing FFL(Firefly luciferase
) specificly were developed by obtaining hybridoma cell lines from the immunized animals injected with the firefly luciferase
The luciferase activities were evaluated through Dual Luciferase Reporter Assay System (Promega) which was normalized by dividing firefly luciferase
activity with that of Renilla luciferase activity.
Many yeast based detection systems involve either the colorimetric detection [24, 29] or the firefly luciferase
The PCR product was inserted into the pGL3-Basic Vector (Promega), which contained a firefly luciferase
Their work, "Designing Quantum Rods for Optimized Energy Transfer with Firefly Luciferase
Enzymes," was published online May 23 in Nano Letters and is forthcoming in print.
The scientists combined a protein obtained from firefly luciferase
with a special dye that allows the protein to emit near-infrared light.
The use of firefly luciferase
reporter genes in a number of intracellular microorganisms including Mycobacterium tuberculosis (49) has facilitated antimicrobial drug testing and discovery.