Feulgen method

Feulgen method

A tissue stain used to identify DNA, in which paraffin-embedded tissues are first hydrolysed with HCl, producing apurinic acid, followed by staining with Schiff’s reagent, resulting in a magenta-coloured nucleus.
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The cells were then fixed using Carnoy fixative (ethanol:acetic acid, 3:1) and stained using the Feulgen method. The number of cells in the mitotic phases (including the late prophase, metaphase, anaphase and telophase; n) per total cells (3,000-3,500; C) was determined under light microscope.
The microscope slides were prepared as described in [14], stained with the Schiff reagent based on Feulgen method for 1h30min.
For the analysis of meiotic cells, the testicular follicles were processed by using the squash technique and then stained according to the conventional Feulgen method by using Schiff reactive after an acid hydrolysis with 1N HCL (hydrochloric acid) at 65[degrees]C for 25min (Feulgen & Rosenbech 1924).