cariolise, identificada pela perda completa do material genetico e sua ausencia, nao sendo possivel ver a coloracao da reacao de Feulgen
, indicativa de processo tardio de morte celular (16) (Figura 1).
Histological sections were cut at 5 m thickness and stained with Feulgen
Nuclear and Light Green to measure the maximum thickness of the apical ectodermal ridges.
Mitotic index were determined by the methods of Feulgen
Comparison between the toluidine blue stain and the feulgen
reaction for evaluation of rabbit sperm chromatin condensation and their relationship with sperm morphology.
The abdomen of each larva was opened ventrally with fine needles and stained with the Feulgen
Root tips were hydrolysed with 1N HCL at 60C during 15 mn and stained according to the Feulgen
arcticum complex were then opened to expose the salivary glands and gonads and stained in Feulgen
(Rothfels and Dunbar, 1953).
On the nonsectioned scales the following techniques were applied: Feulgen
for detection of nuclei accoding to Paulete and Becak (1976) and Toluidine Blue for detection of acid regions according to Junqueira and Junqueira (1983).
stain was used to demonstrate DNA contents.
67 Gbp as estimated by Feulgen
Image Analysis Densitometry; Gregory, 2001, 2012).
Onion roots were prepared by Feulgen
reaction and stained with Schiffs reagent.
reaction specifically stained nuclear chromatin; the aldehyde liberated during hydrolysis is from the deoxypentose but not from ribose.