However, the inclusions demonstrate reactivity to the Feulgen
stain, which is used to identify chromosomal material or DNA in cells.
(onion) test prepared by Feulgen
reaction and stained with the Schiff reagent (Fiskesjo, 1985).
The cells were then fixed using Carnoy fixative (ethanol:acetic acid, 3:1) and stained using the Feulgen
The chromosome number was counted following Can and Hatipoglu (1999) with two modifications (roots were hydrolyzed in 1N HCl at 60AdegC for 5 min, root tips were incubated for 1.5 h in Feulgen
densitometry (FD) and flow cytometry (FC) are the two mostly used methods in DNA content estimation.
The microscope slides were prepared as described in , stained with the Schiff reagent based on Feulgen
method for 1h30min.
Several of those studies have employed the biopsy material stained with H&E [1, 2, 4, 5, 9-11] or Feulgen
stain [12, 13] for designing DSSs.
* cariolise, identificada pela perda completa do material genetico e sua ausencia, nao sendo possivel ver a coloracao da reacao de Feulgen
, indicativa de processo tardio de morte celular (16) (Figura 1).
El numero cromosomico 2n=28 obtenido en la presente investigacion para la poblacion estudiada se obtuvo a partir del recuento de 224 celulas en metafase y por tanto se corrobora un 2n=28 encontrado por Sakai (1952), Goldblatt (1982) y Kenton y Heywood (1984) quienes utilizaron tecnicas estandar de squash y tincion Feulgen
y corresponde al de una poblacion tetraploide con un numero basico x=7, reconocido como el predominante en las monocotiledoneas (Raven, 1975; Dahlgren et al., 1985).
Slides were stained using Feulgen
reaction stain and then counterstained with Fast Green stain (13).
Histological sections were cut at 5 m thickness and stained with Feulgen
Nuclear and Light Green to measure the maximum thickness of the apical ectodermal ridges.
Mitotic index were determined by the methods of Feulgen
. The cells were processed with 1 N HCl at room temperature for 1 minute and then hydrolyzed with 1 N HCl for 10.5 minutes at 60[degrees]C after treated with Feulgen