[4.] Adylkanova S (2010) Selective and Genetic Aspects of Improving the Saryarka and the Degeress Fat-tailed Sheep Breeds in Kazakhstan, Thesis of Doctor of Agricultural Sciences, KazNAU: Almaty, pp.
[8.] Sadykulov T, Smagulov D (2014) Growth and development of young coarse-wooled fat-tailed sheep of various genotypes.
In order to have a relative deeper comprehensive understanding on the germplasm hereditary character and other aspects of this species we selected five microsatellite markers to study the DNA polymorphism of fat-tailed sheep aiming to understand its genetic diversity and provide some beneficial references for further increasing the conservation breeding.
40 fat-tailed sheep in Pingdingshan Henan Province were selected to collect 10ml blood from jugular vein which was processed by ACD anti-freezing (1:6) and stored under -20oC.
All these findings confirmed that the fat-tailed sheep population belongs to the genetic population with high polymorphism information content.
This indicated that the studied fat-tailed sheep population possesses high heterozygosity and richer genetic diversity.
Figure 1 shows 750 bp amplified band of LHss of large-ailed han sheep, small tail han sheep, Yuxi fat-tailed sheep, Lanzhou large-tailed sheep and Mongolian sheep, which coincided with 800 bp in the target band.
Three SNP sites of LHb gene were detected in Lanzhou large-tailed sheep, small-tailed han sheep, Mongolian sheep, large-tailed han sheep and Yuxi fat-tailed sheep. These were C551T site (C/T), G391A site (G/A) and G394A site (G/A), respectively.
Two allele frequencies were 0.811/0.189, 0.868/0.132, 0.594/0.406 and 0.840/0.160, respectively, in small-tailed han sheep, Yuxi fat-tailed sheep, Lanzhou large-tailed sheep and Mongolian sheep.