The LN biopsies broadly expressed the Fas receptor, which was mainly detected along the tubules, in glomerular endothelial cells and in the mesangium.
Fourth, the broad expression of Fas receptor in LN biopsies correlated with apoptotic features.
Taking into account the fact that different genes participate in SLE, transcriptional regulation of the Fas receptor in SLE seems to be activated by IFN-[gamma] .
Regarding the two forms of Fas receptor, we should note that both normal Fas and the sFas transcripts are derived by the same gene promoter; in the case of sFas transcript it results from an alternative splicing that truncates the transcript and results in a protein that lacks intracellular and transmembrane domains (sFas).
Table 1 List of various pro-apoptotic and anti-apoptotic factors involved in the sepsis Pro-apoptotic factors Anti-apoptotic factors BAX Bcl-2 Bcl-xs Bcl-xl, Bcl-Al BIM, PUMA, NOXA Inhibitor of apoptosis proteins FAS ligand Mcl-1 Tumour necrosis factor- a Ras factor- a BID, BAK Apoptosis-inducing factor p53 BAX=Bcl-2-associated X protein, Bcl=B cell lymphoma, BIM=Bcl-2-interacting moderator of cell death, PUMA=p53- upregulated modulator of apoptosis, NOXA=phorbol-12 myristate-l-acetate-induced protein, FAS=cell surface Fas receptor
also known as CD95L, Mcl=M cell lymphoma, Ras=rat sarcoma, BID=BH3 interacting death domain, BAK=Bcl-2 homologous antagonist killer.
Effect of Pcy on cell surface and mRNA expression of Fas receptor in SW480 and SW620 cells.
Pcy treatment of SW620 cells caused a 22-fold (after 24 h) and 35-fold (after 48 h) increase of the Fas receptor transcripts, as measured by real time RT-PCR when compared to untreated cells (Figure 1C).
The Fas receptor agonist (CH-11), when used as a single drug, caused DNA damage in SW480 cells but not in the metastatic SW620 cells.
To investigate the correlation between Fas receptor expression and apoptosis, cells were pre-treated with blocking anti-Fas ZB4 for 1 h before DMSO (control), Pcy and/or agonist anti-Fas CH-11 treatments for 48 h.
We investigated the involvement of the Fas receptor pathway in the Pcy-induced apoptotic response of human colon adenocarcinoma SW480 cells and their derived metastatic SW620 cells.
We observed that untreated SW480 cells expressed Fas receptor at a higher level than for SW620 cells in which the expression of the Fas receptor was only marginal, agreeing with previous reports (8,14).
Nagata, "The many roles of FAS receptor
signaling in the immune system," Immunity, vol.