((a) and (b)) IR of Fas and the Fas ligand
, which are the first step in the FADD-caspase-8 extrinsic pathway, did not change in the different GSK-3[beta] inhibitor- treated groups.
Expression of Fas (CD95) and Fas ligand
on peripheral blood mononuclear cells in critical illness and association with multiorgan dysfunction severity and survival.
Uninfected T cells are known to undergo apoptosis via the cross-linking of CD4 molecules by gp120 secreted out of infected cells and activation or by the binding of Fas ligand
to the Fas antigen (3) These events lead to activation of a family of enzymes, nuclear condensation and DNA fragmentation in the cell.
The structure of TNF-[alpha] is similar to that of the Fas ligand
; consequently, TNF-[alpha] shares some of the functions of Fas ligand
, such as promoting apoptosis.
"It confirms the importance of Fas ligand
" and identifies a control factor for the molecule's production in trophoblasts, he adds.
The following reagents were used for cell labeling in multiparameter flow cytometric analysis (FACS Calibur, BD Biosciences): PE or FITC-conjugated anti-mouse CD4 (clone: RM4-5, Rat IgG2b) Alexa 648 or FITC-conjugated anti-mouse CD3 (clone: 17A2, rat IgG2b), Alexa 648 or FITC-conjugated antimouse-CD8a (clone: 53-6.7, rat IgG2a), PE-conjugated anti-mouse-CD49b (clone: DX5, rat IgM), FITC- or PE-conjugated anti-mouse-CD62L (clone: MEL-14, rat IgG2a), and FITC- or PE-conjugated anti-mouse-CD178 (Fas ligand
) (clone: MFL3, hamster IgG1); all are from BD Biosciences.
Miller, "Fas ligand
is positioned in mouse uterus and placenta to prevent trafficking ofactivated leukocytes between the mother and the conceptus," Journal of Immunology, vol.
in 1998.[sup] It binds to Fas ligand
(FasL) and inhibits FasL-induced apoptosis.
(FasL, CD95L, and TNFSF6) and its receptor Fas (CD95, TNFRSF6) are other members of the TNF superfamily involved in the pathogenesis of IBD.
It also cleaves various non-ECM bioactive molecules such as Fas ligand
, pro-tumor necrosis factor [alpha], E-cadherin, [beta]4-integrin, insulin-like growth factor binding protein 3 and insulin-like growth factor binding protein 5, and [alpha]-defensin.
Stress-induced Fas ligand
expression in T cells is mediated through a MEK kinase 1-regulated response element in the Fas ligant promoter.
Release of preformed Fas ligand
in soluble form is the major factor for activation-induced death of Jurkat T cells.