Chromosomal instability in Suffolk breed was manifold in 5 mg/ml FUdR treated chromosomes.
Genomic instability following 5mg/ml FUdR treatment in Suffolk breed was also recoreded to be exceptionally higher for breed comparison exposed to different environmental condition.
The scale of variation for AC and NoA means in control cultures of Suffolk was high enough to be comparable with FUdR treated cultures from Lohi breed.
However, FUdR treated chromosomes in the two breeds revealed 29 and 78 autosomal bands respectively.
The data on spontaneous and FUdR induced lesions was also subjected to linear regression analysis.
Significant variation in cumulative and per animal in the control and FUdR cultures in the Lohi sheep was in agreement with the findings of similar studies reported elsewhere.
However males were shown to have significantly higher means for both AC and NoA in FUdR cultures (Table 2).
Distribution of spontaneous and FUdR induced lesions and identification of fragile sites in the Lohi genome
Chromosomal band data obtained in the control and FUdR cultures showed 7 lesions mapped to individual chromosomal bands in the control cultures.
In addition the quality of G-bands was poor in Lohi chromosomes especially with FUdR treated cells.
Distribution comparison of spontaneous and FUdR induced lesions between males and female groups
Comparison of the distribution of chromosomal lesions between Lohi females and males did not reveal any conclusive information, probably due to smallness of the data owing to the remarkable stability to cytogenetic damage both in the control and FUdR cultures of Lohi sheep.