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We used the same calibrator for the HMW Adiponectin ELISA after we corrected for the proportion of HMW adiponectin, as measured by FPLC analysis.
Likewise, samples were also subjected to anion exchange chromatography using Mono Q 5/50GL FPLC column (Amersham Biosciences, UK) equilibrated in 20mM imidazole-HCl buffer, pH6.5 containing 1mM CaCl2 and 0.5mM MgCl2 (buffer A).
There was no trypsin inhibitor in F.C peak 4a, FPLC peak 4a and FPLC peak 4b[cents] (Gel D).
The loss of hK5 in the flow-through during SAX FPLC was attributable to incomplete capture of hK5 by the SAX column.
To examine specificity, we prepared 25 lipoprotein fractions by FPLC of plasma of 2 patients with type IIa hyperlipidemia [total TG, 1.75 mmol/L (136 mg/dL)] and type IV hyperlipidemia [total TG, 3.50 mmol/L (310 mg/dL)], respectively.
Fast protein liquid chromatography (FPLC) separation was performed on an ActaPrime apparatus (Amersham Pharmacia Biotech).
The polysaccharide was then dissolved in 10 mM Tris-HCI buffer (pH 8.4) for fast performance liquid chromatography (FPLC) analysis.
We attempted to identify cTnI forms (free, complexed, and degraded cTnI) released in four different patients by use of fast protein liquid chromatography (FPLC; Pharmacia) with prepacked Superdex 200 HR 10/30 columns (separation range, 10-600 kDa), but this method does not differentiate phosphorylated or oxidized cTnI forms.
Initial measurements of HDL-C concentrations before and 30 min after the start of the infusion gave divergent results when we compared results obtained with a polyethylene glycol/sulfated [alpha]-cyclodextrin-based method (Roche HDL Plus) to those obtained with separation of HDL by size-exclusion chromatography by fast protein liquid chromatography (FPLC) with online determination of cholesterol concentration (3).
The performance of only one CDT assay [HPLC or fast protein liquid chromatography (FPLC)] was associated with gender (P = 0.05).
For these purposes, fast protein liquid chromatography (FPLC) has been used to isolate HDL and to measure its cholesterol content (HDL-C) (5).
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