Congenital athymia is a rare and deadly condition associated with complete DiGeorge Anomaly, CHARGE syndrome, and FOXN1
Detection of the gene expression of FoxN1
, FoxE1, and FoxI3 in skin hair follicles of Inner Mongolia cashmere goats: Realtime quantitative polymerase chain reaction (qPCR) detecting system was used to quantify the expression levels of the genes FoxN1
, FoxE1, and FoxI3 and mRNA in skin samples from cashmere goat collected at different fetal and adult stages.
Although thymocytes possess their own transcription factors (TFs) to control their autonomous activities, many thymic activities during thymic development and aging can be regulated by known TFs in TECs, such as the p63 and FoxN1
Effect of boric acid supplementation of ostrich water on the expression of Foxn1
That gene technique involves a "transcription factor" with the unusual name of "forkhead box N1," or Foxn1
. Declining natural levels of Foxn1
have been implicated as a major cause of loss of thymus tissue with aging.
The researchers targeted a protein produced by cells of the thymus -- called FOXN1
-- which helps to control how important genes are switched on.
A gene, called Foxn1
, naturally shuts down as the thymus ages and the team tried to bring it back to youthful levels.
Gawronska-Kozak, "Scarless skin wound healing in FOXN1
deficient (nude) mice is associated with distinctive matrix metalloproteinase expression," Matrix Biology, vol.
To validate microarray data, we analysed SFN, FOXN1
, Krt15, LHX2, CD34, and SOX9 gene expression by using mouse-predesigned oligos (Assay IDs: Mm.PT.58.42262048.g, Mm.PT.58.13135783, Mm.PT.58.5528981, Mm.PT.58.6480133, Mm.PT.58.8626728, and Mm.PT.58.42739087, resp., IDT[R]) and K6b-designed oligos (5'-CATCAAATACACCACCAGCG-3' (forward) and 5'-AAGCAGCCAAAAAGAGAAGC-3' (reverse)).
The genotypes of each sample are represented by blue diamonds (homozygous Foxn1rnu), red circles (homozygous for the wild-type Foxn1
allele), or green triangles (heterozygous +/Foxn1rnu).