FoxJ1 contributes to motile cilia formation and colorectal cancer progression [13, 14].
High Levels of FoxJ1 Expression in the Antennae of A.
Tissue expression patterns showed that FoxJ1 is highly expressed in the antennae of A.
Quantification of p63, ki67, and FoxJ1 immunohistochemistry was performed automatically using Tissue Studio and Image Miner software from Definiens (Definiens AG, Munich, Germany).
The expression of p63, Ki67, and FoxJ1 in the culture sections was examined to detect basal cells, proliferating cells and columnar ciliated cells, respectively.
FoxJ1, a marker for ciliated cells, is a transcription factor that regulates cilia-related genes (Brekman et al., 2014).
They are characterized by multicilia on their apical surface and are positive for the nuclear transcription factor FoxJ1
. Lineage-tracing studies document that they are terminally differentiated cells.
The three transcription factors found to be most highly over-expressed in underfed, 38-day-old 6-arm plutei compared to their well-fed 13-day-old counterparts were CREB, Elk, and FoxJ1 (Fig.
Quantitative RT-PCR was used to measure the expression of several of the genes found to be differentially expressed by RNA-Seq: the transcription factors Elk, FoxJ1, CREB (Fig.
Kuo and his colleagues found that the Foxj1
transcription factor, a class of master proteins that turn other genes on and/or off, is critical to instruct ependymal cells to change shape and assemble into pinwheel-like architecture surrounding stem cells.
He and his team have now discovered that Foxj1 was an 'off switch' that told neuronal stem cells to stop reproducing and triggered the development of a stem cell "niche" in the olfactory bulbs.
Instead, only about three percent of the olfactory neurons came from the Foxj1 stem cells.