The information regarding the age, sex, breed of animal, vaccination against FMDV
and husbandry practices was recorded on a pre-designed proforma.
The general objective of this research was to determine the seroprevalence of FMDV
in buffaloes (Bubalus bubalis) from Jesus Maria Semprun and Catatumbo Counties, Zulia State, Venezuela.
In order to perform GWAS study for identifying FMDV
resistant loci in cattle, we collected a total of 96 female Holstein (26 infected and 70 resistant animals) reared in four field dairy farms which have been affected by FMD and some of the animals were found to be infected (Table 1).
2009), 1F/ 1R primer pair was found 31% superior for primary diagnosis of FMDV
all serotypes than universal primer set P1/P2 also in goats from original field materials.
Was the outbreak caused by the FMDV
used to challenge vaccinated animals, either the strain from Porton Down or the virulent strain resulting from serial passage in pigs which has escaped from one of the labs?
And, because only a portion of the FMDV
genetic information is required to generate a vaccine, the RP-based approach will allow for the differentiation of infected from vaccinated animals (DIVA) when used with current and next generation FMD serology-based diagnostic assays, which is very important in the event of an outbreak.
These tests measure the quantitative antigenic differences between FMDV
structural protein antibodies and thereby estimating the homology between a field isolate and a vaccine strain (Tekleghioghis et al.
The vial containing the virus suspension (106 units of TCID50) was mixed with either of the adjuvant such as aluminum hydroxide gel (AHG), OB or lanolin base (LAN) as described by Dalsgaard (1987) for preparation of adjuvant containing FMD vaccines such as (5) BEI-AHG- FMDV
with 10-6 units of TCID50 F, 4-8 0C, (6) BEI-LAN-FMDV
with 10-6 units of TCID50 F, 4-8degC, (7) BEI-OB-FMDV
with 10-6 units of TCID50 F, 4- 8degC.
Lack of causative FMDV
serotype in the in-use vaccine, poor information on the prevalence of FMDV
serotype in the country, inadequate quantity of immunogen per dose of vaccine, lack of cold chain system, prevailing concurrent diseases and biological and physicochemical immuno-suppressive agents are incriminated to be cause of failure of immuno- prophylaxis (Meyer and Knudersen, 2001).
Seven serotypes of FMDV
are known; serotypes O and A are widely distributed, and the Southern African Territories (SAT) serotypes (1, 2, and 3) usually are restricted to Africa.
However, further increase in FCS in GMEM didn't improve the replication of FMDV
and hence didn't show significant increase in the infectivity titer of the virus.
Differentiation of foot-and-mouth disease virus infected animals from vaccinated animals using a blocking ELISA based on baculovirus expressed FMDV
3ABC antigen and a 3ABC monoclonal antibody.