Encouraged by these validation studies, researchers began to apply HTS miRNA-seq to
FFPE specimens [84, 85].
Nonfrozen, formalin-fixed specimens or
FFPE blocks are the preferred sample type for histopathologic evaluation and immunohistochemistry, and RT-PCR can be performed on either fresh frozen or formalinfixed specimens.
In contrast to 16S specimens, ITS had a much larger proportion of
FFPE tissues.
Several commercial kits specifically designed for nucleic acid extraction from
FFPE tissue specimens have been recently introduced to the market and are gradually being used in research on
FFPE (11, 12).
Of the 50
FFPE tissue blocks evaluated, 43 (86%) were cHL and 7 (14%) were NLPHL.
high-risk profiles were obtained, irrespective of whether fresh tumour biopsies or
FFPE tissue was used.
To help control for the variability encountered in other genomic studies, the present study performed microarray analysis utilizing
FFPE stored liver tissue among a select group of patients, mostly male Caucasians with an average age of 55 years.
Rubicon's TransPLEX RNA kits and GenomePLEX[R] DNA kits are members of its OmniPLEX[R] family of amplification technologies that are designed to deliver sufficient quantities of high quality nucleic acids to enable analyses of
FFPE samples for biomedical research and clinical medicine.
For DNA preparation from
FFPE tissues, 8-10 consecutive sections were subjected to deparaffinization, tissues were then lysed by proteinase K, and DNA was purified by phenol-chloroform extraction.
This partnership enables researchers in India, for the first time, to conduct gene expression profiling from a wide range of clinically relevant sample types, such as small, compromised, or formalin-fixed, paraffin-embedded (
FFPE) tissues.
Until now, researchers thought that this formalin-fixed, paraffin-embedded (
FFPE) preservation process destroyed, modified, or degraded the nucleic acids, specifically the DNA and RNA, in biopsy samples, precluding gene expression analysis.