Encouraged by these validation studies, researchers began to apply HTS miRNA-seq to FFPE
specimens [84, 85].
Nonfrozen, formalin-fixed specimens or FFPE
blocks are the preferred sample type for histopathologic evaluation and immunohistochemistry, and RT-PCR can be performed on either fresh frozen or formalinfixed specimens.
In contrast to 16S specimens, ITS had a much larger proportion of FFPE
Several commercial kits specifically designed for nucleic acid extraction from FFPE
tissue specimens have been recently introduced to the market and are gradually being used in research on FFPE
Of the 50 FFPE
tissue blocks evaluated, 43 (86%) were cHL and 7 (14%) were NLPHL.
high-risk profiles were obtained, irrespective of whether fresh tumour biopsies or FFPE
tissue was used.
To help control for the variability encountered in other genomic studies, the present study performed microarray analysis utilizing FFPE
stored liver tissue among a select group of patients, mostly male Caucasians with an average age of 55 years.
Rubicon's TransPLEX RNA kits and GenomePLEX[R] DNA kits are members of its OmniPLEX[R] family of amplification technologies that are designed to deliver sufficient quantities of high quality nucleic acids to enable analyses of FFPE
samples for biomedical research and clinical medicine.
For DNA preparation from FFPE
tissues, 8-10 consecutive sections were subjected to deparaffinization, tissues were then lysed by proteinase K, and DNA was purified by phenol-chloroform extraction.
This partnership enables researchers in India, for the first time, to conduct gene expression profiling from a wide range of clinically relevant sample types, such as small, compromised, or formalin-fixed, paraffin-embedded (FFPE
Until now, researchers thought that this formalin-fixed, paraffin-embedded (FFPE
) preservation process destroyed, modified, or degraded the nucleic acids, specifically the DNA and RNA, in biopsy samples, precluding gene expression analysis.