FEN1

FEN1

A gene on chromosome 11q12 that encodes a protein belonging to the XPG/RAD2 endonuclease family, which removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging-strand DNA synthesis. Direct physical interaction between FEN1 and AP endonuclease 1 during long-patch base excision repair provides co-ordinated loading of the proteins onto the substrate, passing the substrate from one enzyme to another.
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Unlike single-patch repair, the long-patch repair is PCNA-dependent pathway (Frosina, 1996) in which the involvement of DNA polymerase-[beta] and DNA polymerase [delta]/[epsilon] as well as the FEN1 endonuclease adds several nucleotide to remove the displaced DNA flap and DNA ligase1 for sealing the backbone of the DNA double helix.
We found that genes required for the replication of chromosomal DNA (PCNA, FEN1, CDC6, MCM2, MCM3, MCM4, MCM5, ORC1, ORC6, RRM1, RRM2) and genes required for postreplicative phases of the cell division cycle (e.
The team has also used the technique to analyze functions of the p53 gene (a tumor suppressor that is the most commonly mutated gene in human cancers); to study the human FEN1 gene (important in DNA replication and repair); and to evaluate the stability of DNA sequences from the human genome.