FEN1

FEN1

A gene on chromosome 11q12 that encodes a protein belonging to the XPG/RAD2 endonuclease family, which removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging-strand DNA synthesis. Direct physical interaction between FEN1 and AP endonuclease 1 during long-patch base excision repair provides co-ordinated loading of the proteins onto the substrate, passing the substrate from one enzyme to another.
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The FEN1 gene product is a structure-specific metallonuclease best known for its essential roles in the penultimate steps of Okazaki fragment maturation and long-patch base excision repair.
DNA repair array gene expression results showed that when this patient's results compare with the other NF1 patients without malignancy; ATR, CCNO, MLH1, NEIL1, NTHL1, RAD18, RAD 51, XRCC1, XRCC3 genes are overexpressed and ATM, BRCA1, BRCA2, ERCC4, EXO1, FEN1, LIG1, LIG3, MPG, MRE11A, MSH2, NEIL2, PMS1, POLD3, RFC1, SMUG1, TDG, TOP3B genes are down-regulated in this patient (mother).
The rats were randomly divided into six groups (n=20 for each group): sham operation group (S group), I/R group, normal saline I/R group (NS group), fentanyl low dose group (Fen1 group, 2 [micro]g/kg), fentanyl middle dose group (Fen2 group, 4 [micro]g/kg), and fentanyl high dose group (Fen3 group, 6 [micro]g/kg).
To knock down expression of PCNA or Fen1 the respective plasmids to produce short hairpin RNA of PCNA or Fen-1 in pLKO.1 were obtained from National RNAi Core Facility (Genomic Research Center, Taipei, Taiwan).
Xu, "Curcumin inhibits proliferation of breast cancer cells through Nrf2-mediated down-regulation of Fen1 expression," Journal of Steroid Biochemistry and Molecular Biology, vol.
Zhou et al., "Functional FEN1 genetic variants contribute to risk of hepatocellular carcinoma, esophageal cancer, gastric cancer and colorectal cancer," Carcinogenesis, vol.
Em celulas do adenocarcinoma mamario (MCF-7), Cheng e colaboradores demonstraram que a proliferacao celular desta linhagem depende de Fen1, uma das enzimas envolvidas na replicacao e reparacao do DNA, e que diminui apos exposicao ao seu RNA de interferencia (siRNA).
Flap endonuclease 1 (Fen1 in archaea or FEN-1 in eukaryotes) plays roles in both DNA replication and repair, often in conjunction with PCNA.
The xeroderma pigmentosum group G (XPG) gene encodes an 1186-amino acid structure-specific endonuclease, known as XPG gene, which is an indispensable component of NER and belongs to the flap structure-specific endonuclease 1 (FEN1) family.5 This protein also has a role in other cellular processes, which is involved in the RNA polymerase II transcription and transcription-coupled DNA repair.8.9 Previous experimental studies suggested that defective XPG gene polymorphisms have a vital role in the development of cancer, mainly due to DNA repair defects, genomic instability and failure of gene transcription modulation.10
Unlike single-patch repair, the long-patch repair is PCNA-dependent pathway (Frosina, 1996) in which the involvement of DNA polymerase-[beta] and DNA polymerase [delta]/[epsilon] as well as the FEN1 endonuclease adds several nucleotide to remove the displaced DNA flap and DNA ligase1 for sealing the backbone of the DNA double helix.
Interactions between APE1 and DNA polymerase [beta] or flap endonuclease 1 (FEN1) induce the removal of the remaining abasic site at the 5'-end of the cleavage site after excision of the AP site by APE1 [31, 32].
We found that genes required for the replication of chromosomal DNA (PCNA, FEN1, CDC6, MCM2, MCM3, MCM4, MCM5, ORC1, ORC6, RRM1, RRM2) and genes required for postreplicative phases of the cell division cycle (e.g., CCNB1, PLK1) are coordinately induced and reach maximal expression levels between 8 and 24 hr (Figure 6B), consistent with the timing of the histologic changes observed in Figure 6A.