A gene on chromosome 3p26 that encodes a protein of the Fanconi anaemia complementation group (here, complementation group D2), which co-locates with other proteins—e.g., BRCA1 and BRCA2—and is involved in homology-directed DNA repair.
In comparison, enzymes in concern with fatty acid modification and oil accumulation encountered the most significant regulation, with oleate desaturase (FAD2), linoleate desaturase (FAD3), oleateD12-hydroxylase (FAH12, only expressed in Jatropha) and D12 fatty acid conjugase (FADX, only expressed in Vernicia) identified for this category.
Previous work has proved successive fatty acid desaturations in various oil plants, including the stearic acid to oleic acid conversion catalyzed by SAD, subsequent linoleic acid formation by FAD2, as well as a-linoleic acid or a-eleostearic by FAD3 and FADX, respectively.
For example, three different microsomal [DELTA]12 oleate desaturase genes (FAD2s) were reported for sunflower (Martinez-Rivas et al., 2001), and two microsomal FAD2s were reported each for soybean (Heppard et al., 1996) and for cotton (Liu et al., 1997), while three different microsomal [omega]-3 desaturase genes (FAD3s) were reported for soybean (Bilyeu et al., 2003).
Because HO2 only marginally increased the C18:1 content of the seed oil but significantly increased leaf and root C18:1 contents it is unlikely that the second mutation also affected fad2. The increase of C18:1 in the leaves indicates, that fad6, the plastidial oleic acid desaturase, might be affected by HO2.