GLE affected F-actin
formation and distribution induced by TGF-[beta]1 stimulation
Specifically, shrunken cells and F-actin
accumulation were observed after nicotine treatment.
d) Fluorescence microscopy observed the increased H9c2 cells surface area of F-actin
staining in PA group, which was suppressed by Nec- 1 (10 nM), according to the semiquantitative results by ImageJ software, n = 3.
, filamentous actin; FITC, fluorescein isothiocyanate; FSW, filter-sterilized seawater; LPS, lipopolysaccharide; MMP, matrix metalloproteinase(s); mPBS, marine phosphate buffered saline; TCT, tracheal cytotoxin.
Unipolar reorganization of F-actin
layer at bacterial division and bundling of actin filaments by plastin correlate with movement of Shigella flexneri within HeLa cells.
As cell migration is related to reorganization of the actin cytoskeleton, we also measured the cytoskeletal actin organization by staining F-actin
with phalloidin in PC-3 cells and DU145 cells.
25% bovine serum albumin for 30 min, and stained with phalloidin and DAPI solution to visualize F-actin
and nuclei, respectively (Life Technology, Seoul, Korea).
Focal adhesion kinase is a regulator of F-actin
dynamics: New insights from studies in the testis," Spermatogenesis 3(3): e25385.
Prior to neural tube closure, F-actin
is accumulated at the medial plane of the epidermal cells adjacent to the neural plate.
Next, the study looked at F-actin
filaments, a protein structure which previous studies have shown plays a role in the constriction of ASM, and found that 6-shogaol was effective in speedily dissolving these filaments.
vc]) covalently binds to G-actin and F-actin
, and causes actin depolymerization, which leads to cell rounding (Kudryashov et al.
Finally, to test whether the anti-actin ELISA used was specific for F-actin
, we assayed for IgG anti-F-actin
in 18 sera from patients with type 1 AH, positive by IF for IgG anti-F-actin
on HEP-2 cells (titer range, 1:160 -1:1280), and 30 negative sera from healthy controls.