To better understand the molecular mechanisms underlying the deleterious effects of COQ6, Western blot analysis of nephrin and F-actin
was conducted, and the expression levels of both proteins were significantly lower in the si-RNA-COQ6 group than in the control group [Figure 2]b and [Figure 2]c.
Further experimental studies are required to explore the function of LOOP-1 in the binding of plastin-3 to F-actin
Caption: Figure 4: Immunostaining of F-actin
in RANKL-induced BMMs osteoclastogenesis treated with miR-16-5p inhibitor or mimics.
Alexa Fluor[R] 555 phalloidin was used to stain F-actin
. Fluorescent images were examined using a Leica TCS SP5 confocal microscope.
Fluorescence microscopic studies showed the effect of nicotine (1 uM and 10 uM) on F-actin
distribution in the A549 cells.
A Cluster of Basic Repeats in the Dystrophin Rod Domain Binds F-actin
through an Electrostatic Interaction.
Two protein spots demonstrating reduced expression by HG, identified as the actin capping proteins, F-actin
capping protein [beta]-subunit, and actin-like protein 3.
"Focal adhesion kinase and actin regulatory/binding proteins that modulate F-actin
organization at the tissue barrier; Lesson from the testis," Tissue Barriers 1(2); e24252.
He said that they have found that they had massive impairment of craniofacial skeletal development, however, this was fully restored when they added human PLS3 and the same thing happened when we added two other proteins, actinin 1 and actinin 4, F-actin
proteins2 which are involved in 'bundling' or building the 'scaffolding' for cells, and it seems that these proteins can compensate for the loss of PLS3.
When viewed at the apical surface, F-actin
labeled (rhodamine phalloidin) epithelial cells of non-morphogenic appendages were shaped like flat tiles; the epithelium was uniform in appearance, and each cell contained a distinctive peripheral band of actin adjacent to the apical cell border (Fig.
The actin microfilaments distribution was examined using phalloidin, which specifically binds to polymerized actin or F-actin
. Control cells (Figure 6(a)) show typical microfilament bundles in the cytoplasm towards the plasma membrane.
The C-terminal domain can mediate the link between the actin cytoskeleton and the plasma membrane through F-actin
binding site .