An inventory of 1152 expressed sequence tags
obtained by partial sequencing of cDNAs from Arabidopsis thaliana.
A total of 5,019 expressed sequence tags
(ESTs) were sequenced, which resulted in the identification of 620 contigs and 1,151 singletons.
There, fast, state-of-the-art gene-sequencing instruments will identify up to a half-million switchgrass sequence fragments, called "expressed sequence tags
" (ESTs), within the next 3 years.
In the current era of "single nucleotide polymorphisms," "expressed sequence tags
," and "functional genomics," it is all too easy to forget that for the majority of this century the organization and even existence of plant genes were inferred from phenotype, not the other way around.
KEY WORDS: expressed sequence tags
, simple sequence repeats, linkage mapping, population genetics, oyster, Crassostrea virginica
We report here on our strategy to develop polymorphic markers using expressed sequence tags
(ESTs) by designing primers flanking single or multiple SSRs with three or more repeats.
To unravel the code the sugar beet blueprint is written in, we need what Mitch McGrath--a geneticist at the ARS Sugar Beet and Bean Research Unit at East Lansing, Michigan--calls an "alphabet soup" of genetic tools, such as ESTs (expressed sequence tags
), BACs (bacterial artificial chromosomes), and RILs (recombinant inbred lines).
A paper by Yamamoto and Sasaki covers the Rice Genome Research Program's characterization of copy DNA clones (cDNAs) of expressed genes using expressed sequence tags
(ESTs) and the application of cDNAs for construction of physical maps and cataloguing the rice genome.
Expressed Sequence Tags
from cDNA & MPSS Libraries
That's very specific, and expensive, research." It recently yielded about 20,000 sugar beet gene indicator tags--ESTs(expressed sequence tags
)--which represent some, but not all, of the 30,000 genes thought to make up the functional part of the genome.
Researchers in Miami will then work toward identifying the genes involved in disease resistance reactions, using sophisticated techniques called expressed sequence tags
and microarray technology.
The aim of this study was to characterize a set of novel expressed sequence tags
(EST)-derived microsatellites that have more conserved flanking regions so that some microsatellites can achieve cross-species amplification and good transferability in other related bivalve species (Bouck & Vision 2007).