The crude n-hexane extract (13 g) was subjected to silica gel column chromatography and the column eluted with either one or a mixture of two of n-hexane,
ethylacetate and methanol.
and reconstituted in 1ml
ethylacetate for analysis on
Preliminary experiment with a number of organic solvents commonly used (benzene,
ethylacetate, methylene chloride, and ethylene chloride) for the ion-pair extraction was studied.
[76] isolated five novel cyathane diterpenes, identified as cyathins DH (1-5), in addition to three well known diterpenes, namely, neosarcodonin, cyathatriol, and 11-O-acetylcyathatriol from the
ethylacetate extract of Cyathus africans.
A mixture (1:1) of
ethylacetate and n-hexane was placed in separating funnel containing 10 mL aqueous solution of parathion.
Contents of sweep net were placed in
ethylacetate kill jar until the arthropods were dead, then the insect material was transferred to labeled plastic bottle and preserved in 70% ethanol.
The benzene, hexane,
ethylacetate, methanol and chloroform leaf extract of Andrographis paniculata was found to be more effective against Cx.
Sung, 2004.Antimutagenic, antioxidant and free radical scavenging activity of
ethylacetate extracts from white, yellow and red onion.
Identification and quantification was based on external calibration with standard solutions of acetaldehyde, 2-pentanone, 2-butanone,
ethylacetate (Fluka, Buchs SG, Switzerland); ethanol (Arcus, Oslo, Norway); 2-methyl-1-propanol, 2-methyl-1-butanol, 2-methyl-1-butanal, 3-methyl-1-butanol, 2-methyl-1-butanol, 2-methyl-1-propanol, 3-methyl-1-butanal, 3-methyl-1-butanol, 2-methyl-1-butanol, 2-methyl-1-propanal, diacetyl, 2-propanol, 1- butanol, 2-butanol, acetoin, iso-butylacetate, acetone, 2,3-pentanedione,
ethylacetate, acetone, isobutyl acetate and ethyl butyrate (Merck KGaA, Darmstadt, Germany).
Further chromatographic fractionation of PSB (3.48 g) followed by repeated recrystallization in petroleum ether/
ethylacetate 10:3 afforded 3.1 g of (1) mp 248-249[degrees]C and [R.sub.f] = 0.76 (toluene/ethyl acetate 10:1).
Six fraction were obtained (fraction A, 100% n-hexane), (fraction B, n-hexane:ethylacetate, 60:40), (fraction C, n-hexane:ethylacetate,20:80), (fraction D,
ethylacetate::methanol 80:20), (fraction E,
ethylacetate:methanol, 60:40), (fraction F, 100% methanol).
Trianthema Parts Used Extract yield % % Inhibition decandra extracts (w/w) Petroleum ether Leaves 2.56 20.75 extract Chloroform extract Leaves 1.44 60
Ethylacetate extract Leaves 1.36 47.48 Table 2: [alpha]-Amylase inhibition activity of the extracts tested by Pre-incubation Method.