In order to determine the growth pattern, 1 ml of fungal spore suspension was inoculated in 250 ml Erlenmeyer flasks
containing 100 ml of Czapek Dox broth with and without monocrotophos (500 mg/L).
In the subsequent experiments, the samples were treated with 50% NH3 solution followed by refluxing on water bath for 6 hours in Erlenmeyer flasks
. In addition, the samples were also treated with papaya (2.5 g) in a water bath for 2 hours to check the catalytic effect on the hydrolysis of carbohydrates.
Inoculum (25 ml) was prepared in 100 ml Erlenmeyer flasks
containing 30 g [l.sup.-1] xylose, 10 g [l.sup.-1] yeast extract, 20 g [l.sup.-1] peptone, 0.5[gl.sup.-1] [K.sub.2]HP[O.sub.4], 0.5[gl.sup.-1] K[H.sub.2] P[O.sub.4], 2[gl.sup.-1] [(N[H.sub.4]).sub.2] S[O.sub.4], 0.5[gl.sup.-1] MgS[O.sub.4] x 7[H.sub.2]O and pH 6.0 and it was inoculated with 5 ml of preculture and incubated for 24 h on a rotary shaker (200 rpm) at 30[degrees]C.
To investigate desorption efficiency of different eluents, metal laden immobilized cells were filtered and after soaking in filter paper to remove any liquid adhered, these beads were transferred to 50 ml eluents taken in 250 ml Erlenmeyer flask
. Each flask containing eluents solution was incubated for 4 h at 30[degrees]C and 150 rpm.
All the conditions were optimized in Erlenmeyer flasks
(250 mL) containing 25 mL total fermentation medium using one factor at a time approach.
For biosynthesis of nanoparticles, 2.0 mL of the seeds extract was mixed with 20 uL of freshly prepared 1x10-2 M aqueous copper sulphate pentahydrate solution in 250 mL Erlenmeyer flask
under continuous magnetic stirring.
One hundred gram CSM and 500 mL water were transferred to a 1000 mL Erlenmeyer flask
, pH was adjusted to 8, and then 3% alcalase was added to CSM.
were used as the vessel for the analyte, and burets calibrated to tenths of a milliliter were used for the titrant, suspended in an iron stand by a universal clamp.
The prepared solution (50 ml) was added to 250 ml Erlenmeyer flask
, one containing 30 mg of chitosan adsorbent (1.18 mm) and the other without chitosan.
In conclusion, culture media was improved for rGILCC1 laccase production in Pichia pastoris [500 mL Erlenmeyer flask
containing 150 mL culture media, 10% (v/v) inoculum, 1.0 mM CuS[O.sub.4],30 g[L.sup.-1] glucose, 5 mM N[H.sub.4]S[O.sub.4], 10 g[L.sup.-1] peptone, and 5 g[L.sup.-1] yeast extract for 168 h, at 30[degrees] C and 180 rpm] with a laccase activity of 4.7 [+ or -] 0.4 U[L.sup.-1].
fujikuroi using SmF in Erlenmeyer flask
, STR, and BCR.
We pipetted 25 mL of sample solution into an Erlenmeyer flask
(100 mL) with a stopper, and put 25 mL of acetyl acetone--ammonium acetate into the Erlenmeyer flask
and mixed it until homogeneous.