The present paper describes the method of determination of catechin and epicatechin.
Stock solutions of catechin and epicatechin (4 mg/mL) were prepared by dissolving standards into methanol.
Calibration standards were prepared by diluting the catechin and epicatechin stock solutions with methanol in the concentration range of 100-600 ^g/mL.
The peaks for catechin and epicatechin in the samples were confirmed by comparing the retention times of the peak with those of standards.
Despite relatively low amounts of catechin and epicatechin in foods, once absorbed and metabolized they have the potential to affect numerous important health parameters (Keen et al.
Flavanols such as epicatechin are thought to influence mitochondrial function through both activation and control of programmed cell death (apoptosis).
Epicatechin mediates beneficial effects of flavanol-rich cocoa on vascular function in humans.
In a third study presented at the meeting, epicatechin
was added to unroasted cocoa, which was then heat processed.
1%) in drinking water Epicatechin
-3-gallate (24%) Epigallocatechin (5%) Epicatechin
(6%) Caporali et al.
MRM analysis with the mass transitions m/z 291/139 (catechin or epicatechin), 305/139 (methyl catechin or epicatechin), 579/291 (dimers), 579/289 (dimers) and 867/579 (tri-mers) indicated the presence of monomeric catechin or epicatechin, methylated catechin or epicatechin, and proanthocyanidin dimers and proanthocyanidin trimers in urine collected over 16 h after GSE treatment.
Interestingly, the MRM analysis of brain samples indicated the presence of catechin and epicatechin (Fig.
In this study, monomeric catechin and epicatechin were shown to have crossed the blood brain barrier and gained entry to the brain in GSE-treated rats.