We determined NAI 50% inhibitory concentrations ([IC.sub.50]s) of isolates using fluorometric-based NA inhibition assays (5) and evaluated their NA activity ([V.sub.max] [maximum velocity of substrate conversion]) by performing enzyme kinetics
The unit includes monochromator-based individual wavelength selection or wavelength scanning from 200 to 999 nm to accommodate an extensive range of assays, including nucleic acid and protein quantification, ELISA, microbial growth, endotoxin, ROS assays and enzyme kinetics
. The onboard software in Epoch 2 eliminates the need for a separate computer and includes predefined common protocols for rapid assay setup and recall.
Due to a prior lack of adequate quantities of purified enzyme, a systematic study of enzyme kinetics
has not been previously undertaken, though would be required for development of any inhibitor or assay of inhibition.
During the training, Dana learned new techniques such as molecular modelling and docking, enzyme kinetics
and tubulin assay, animals handling and in vivo testing, and performed experiments related to discovering novel therapies for the treatment of breast cancer.
The IC50 values of compounds were calculated by using the EZ-Fit Enzyme kinetics
software program (Perrella Scientific Inc., Amherst, MA, USA).
Similar procedure was performed without inhibitor and enzyme kinetics
The enzyme kinetics
of recombinant endo-[beta]-1,4-glucanase against CMC-Na was investigated.It revealed that the apparent Km value of the enzyme determined from the Lineweaver-Burk plot was estimated to be 64.5 mM.
Figure 1 presents the three phases of enzyme kinetics
explains the catalytic behavior of enzymes.
The mechanism of hMAO-B inhibition was investigated by enzyme kinetics
, following a similar procedure to the MAO inhibition assay.
Segel, Enzyme kinetics
, Wiley, New York, NY, USA, 1975.
The enzyme kinetics
measurement parts were performed in Agri Ibrahim Cecen University Central Research Laboratory.