diphtheriae tox gene), determine their biotypes (2 variant gravis and 12 variant mitis), and characterize their toxin production using the Elek test
(positive for 9 isolates).
However, a modified Elek test yielded a negative result (8).
ulcerans, suggesting a higher sensitivity of cytotoxicity assays than that seen with the Elek test (15).
We determined DT production using a modified Elek test
(6); we used C.
Diphtheria toxin production was evaluated by a modified Elek test
FRC24 is a toxigenic isolate; toxigenicity was confirmed by both tox gene detection and Elek test
Results from use of the modified Elek test
(6) indicated that all feline isolates were negative for production of diphtheria toxin; however, an atypical precipitation was observed after 36 h of incubation.
ulcerans tox-specific PCR (4), and the Elek test
as described previously (4,5).
Toxigenicity was determined by both dtx polymerase chain reaction and Elek test
Easy-to-perform modified Elek test
to identify Shiga-like toxin-producing diarrhoeogenic Escherichia colt.
Toxigenicity status was determined by the Elek test
, as recommended by WHO (9), and by the polymerase chain reaction (PCR), which targeted both A and B subunits of the tox gene (10).
For some isolates, the Elek test
was also performed; [ILLEGIBLE TEXT] negative.