electrochemiluminescence


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electrochemiluminescence

, electrochemoluminescence (ē-lek″trō-kēm″ĭ-loo″mĭ-nes′ĕns) (ē-lek″trō-kēm″ō-loo″mĭ-nes′ĕns)
The production of visible light by an electrochemical reaction in which molecules, after becoming electronically excited, release visible electromagnetic energy when they return to their relaxed state. Electrochemiluminescence is used in laboratory science as a means of measuring the concentration of specific chemicals in solution. Light-emitting molecules that commonly bind to the chemical of interest are introduced into the solution, and the amount of light given off by the “labeled” or “tagged” chemical is measured.
References in periodicals archive ?
The prepared Au electrode was used as work electrode for electrochemiluminescence detection.
Both vitamin B12 and folate levels were estimated by electrochemiluminescence on Roche cobas e411 analyzer using commercial kits (Roche diagnostics KK Tokyo Japan).
Electrochemiluminescence (ECL) is a kind of luminescence produced during elecrochemical reactions in solution.
The ferritin levels were measured via electrochemiluminescence immunoassay (ECLIA) technology (Roche Diagnostics, Basel, Switzerland), and the EPO levels were measured by an improved Immulite enzymelinked immunosorbent assay (ELISA) system (Siemens AG, Berlin, Germany).
Several other prospective assay formats have also been demonstrated, such as those based on electrochemiluminescence, immuno-polymerase chain reaction (PCR), biosensors, cellular-based assay and endopeptidase activity assay.
The serum insulin level was measured by an electrochemiluminescence immunoassay (ECLIA) and the insulin resistance index was calculated according to the homeostasis model assessment (HOMA-IR) which correlates well with the euglycemic hyperinsulinemic clamp in people with diabetes [12].
Prostate-specific antigen (PSA) was assessed using Roche Elecsys total PSA assay (Roche Diagnostic Corporation, Basel, Switzerland) by electrochemiluminescence.
Electrochemiluminescence can be used in a second mode as well, showing the fingerprint as a positive image.
It includes automation, remote data acquisition, enzyme-linked fluorescence assay (ELFA), chemiluminescence immunoassay (CLIA), capillary electrophoreses (CE) based CLIA, electrochemiluminescence immunoassay (ECLIA), direct access testing and rapid accurate quantification at point of care.
Intended as an aid in the in the diagnosis of myocardial infarction, the electrochemiluminescence immunoassay is used for the in vitro quantitative determination of TnT-hs in human serum and plasma.
ALI (Novartis, Switzerland), DOCA (Sigma, USA), primary antibodies against [beta]-actin, extracellular signal kinase 1/2 (ERK1/2), phosphorylated ERK1/2 (PERK1/2) and metalloproteinase-9 (MMP-9) (Santa Cruz, USA), secondary antibodies, two-quinolinecarboxylic acid (BCA) (Beijing Zhongshan Golden-Bridge Biotech, China), electrochemiluminescence (ECL) kit (Pierce), Sirius red (Beijing Haide Biotech, China) and radioimmunoassay kit (Beijing Yuanzi Biotech, China) were used in the present study.

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