Edman digestionA technique used to determine the sequence of a protein’s amino acids.
A peptide of interest is treated with phenylisothiocyanate-Edman reagent, which attaches at the N-terminal residue, making the first peptide bond in the protein susceptible to digestion by a mild acid. The first amino acid is removed and determined by chemical means; repetition on up to 20–30 amino acids can be accomplished automatically by the sequencers.
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