and EAEC (EAST-1 positive strains) was proximal to phylogroup B1, and EHEC was relatively coincident with phylogroup A.
Prevalence of enteric pathogens in children less than 5 years of age (N=237) Diarrhoeal pathogen n (%) Samples positive 140 (59.1%) Positive for only one diarrhoeal pathogen 64 (27.0%) ETEC 7 (3.0%) EAEC 13 (5.5%) Atypical EPEC
2 (0.8%) Typical EPEC
2 (0.8%) EIEC 9 (3.8%) Shigella spp.
Our study is close to study by Arif and Salih (23) in Sulaimani, Iraq, and global reports by Malvi (25) in India, that showed the distribution of atypical EPEC
was higher than typical EPEC
designs and manufactures control systems for mobile work machines, electronics and software for demanding conditions.
Total RNAwas isolated using the RNeasy Plus Mini Kit (Qiagen, Inc., USA) from uninfected IEC-6 cells or cells infected for 3 h with [10.sup.6] CFU per mL of each EPEC
All recovered EPEC
isolates were classified as atypical, due to the lack of bfp plasmidic genes as revealed by negative PCR results .
A GI panel of stool revealed EPEC
. A 3-day course of azithromycin was initiated.
A previously published stepwise evolutionary model showed the acquisition of stx2 by a strain of EPEC
O55:H7, resulting in emergence of a strain of STEC O55:H7, which was [beta]-glucuronidase positive and sorbitol positive, closely related but ancestral to STEC O157:H7, which was [beta]-glucuronidase positive and sorbitol positive (34).
coli strains with virulence genes profiles similar to EHEC, EPEC
, and ETEC in environmental waters has already been reported.
For instance, EPEC
strains E2348/69 and E851/71 caused higher attack rates and more severe ADD, whereas EPEC
strain E74/68 elicited lower attack rates or milder diarrheal diseases or no illness at all [26, 31, 32].
However, many enteric pathogens, such as enteropathogenic Escherichia coli (EPEC
), Shigella, and Salmonella, use various strategies to manipulate the host cell death and to enhance their replication and survival.
Of the 18 DEC isolates, enteropathogenic Escherichia coli (EPEC
) and Shiga-toxin-producing E.