Statistical comparison of the performances of the two media, EMB agar and Hicrome E.
coli produced colonies with typical high metallic sheen on EMB agar and blue-green colonies on Hicrome E.
24 strains were further analyzed by gram staining which show majority of them were gram-ve and rod shape, but one of them was Cocci to confirm it these strains were cultured on EMB agar and KAA agar.
In the present study 24 selected bacterial colonies were detected by Gram staining and by growth of selected isolates on EMB agar and KAA agar.
Loop full of single typical colony was transferred from EMB agar plate to 9ml sterilized MacConkey broth tube containing inverted Durham's tube.
Coliforms appeared in the form of pink colored colonies on the surface of EMB agar.
These
EMB agar plates were incubated for 48 hours and the number of colonies were counted and then multiplied with dilution factor to get total number of colonies.
Nutrient agar, Maconkey agar and
EMB agar were used as primary culture media for isolation of organisms from samples according to methods described by Quinn et al.
Bacterial DNA was extracted easily by boiling method when the isolates were grown on sorbitol MacConkey's agar or
EMB agar at 37oC for 12-24 hrs.
Swabs were plated on
EMB agar and 5% sheep blood agar.
After sterilization, the flask containing
EMB agar medium was placed in laminar air flow cabinet and poured the medium into sterilized petriplates.
Later different species sensitive strain as chosen on
EMB agar.
