Growth of bacteria on nutrient agar occurred after 24 h while on EMB agar
after 48 h.
A amostra B1 coletada na nascente localizada a Comunidade Boiadeiro apresentou resultado positivo para Coliformes Totais no Caldo CBLVB e presenca de Escherichia Coli em placa de EMB Agar
, com numero estimado em 100UFC, tendo apresentado os resultados fisico-quimicos: Alcalinidade Total: 20 mg/ml-1, Cloreto: 20 mg/l-1, Dureza Total: 20 mg/ml-1, Amonia: 0,121 mg/ml-1, Ferro: 0.10 mg/l -1, PH: 6,5 e Cor: 3 mg/l-1.
24 strains were further analyzed by gram staining which show majority of them were gram-ve and rod shape, but one of them was Cocci to confirm it these strains were cultured on EMB agar
and KAA agar.
Loop full of single typical colony was transferred from EMB agar
plate to 9ml sterilized MacConkey broth tube containing inverted Durham's tube.
was isolated, by placing EMB agar
plate in open in room for 20 minutes and then incubating it for 24 hours at 37degC.
Then, 0.1 ml of the container transferred onto blood agar plates and other 0.1 ml onto EMB agar
. The culture plates were incubated at 37[degrees]C for 24-48 hours.
Direkt baki yapildiktan sonra bu sividan %5 koyun kanli agar, EMB agar
ve cikolata agara tek koloni dusurme yontemiyle ekim yapilmistir.
coli, while picked up and streaked upon EMB agar
radioresistens delayed bacterial identification, and it was not until the organism was later observed growing on EMB agar
that an incorrect diagnosis was suspected.
Lactose utilization was verified on EMB agar
or MacConkey agar (Difco) with lactose as the carbon source.
Eight ioslates were confirmed as E.coli, which showed typical greenish metallic sheen on EMB agar
as well as gram negative rods morphologically similar to E.
The growth medium's used during the entire process include Nutrient agar, Macconkey's agar, EMB agar
and Mannitol salt agar