ELISA

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Related to ELISA assay: ELISA test

assay

 [as´a]
determination of the purity of a substance or the amount of any particular constituent of a mixture.
biological assay bioassay; determination of the potency of a drug or other substance by comparing the effects it has on animals with those of a reference standard.
CH50 assay a test of total complement activity as the capacity of serum to lyse a standard preparation of sheep red blood cells coated with antisheep erythrocyte antibody. The reciprocal of the dilution of serum that lyses 50 per cent of the erythrocytes is the whole complement titer in CH50 units per milliliter of serum.
enzyme-linked immunosorbent assay (ELISA) any enzyme immunoassay using an enzyme-labeled immunoreactant (antigen or antibody) and an immunoadsorbent (antigen or antibody bound to a solid support). A variety of methods are used for measuring the unknown concentration, such as either competitive binding between the labeled reactant and unlabeled unknown or a sandwich technique in which the unknown antigen binds both the immunoadsorbent and labeled antibody. One of the uses of ELISA is to screen blood for antibody to the human immunodeficiency virus; a positive result indicates probable exposure to the virus and possibly that the virus is in the blood. Since false-positives can occur, a back-up test is used to confirm positive findings.
 ELISA: With antigen capture ELISA, the wells are coated with antibody to the virus. The sample containing virus (1) is added and, after washing several times, enzyme conjugated to an antibody to the virus is added (2). Finally, after a further cycle of washing, the enzyme's substrate (3) is added. A colored product is formed if the viral antigen is present in the sample (4). From Hart and Shears, 1997.
microhemagglutination assay–Treponema pallidum (MHA-TP) a Treponema pallidum hemagglutination assay using microtechniques; used in the detection of syphilis.
radioreceptor assay a radioligand assay in which a radiolabeled hormone is used to measure the concentration of specific cellular receptors for the hormone in tissue specimens, an example being radioassay of estrogen receptors in breast tissue.
thyroid-stimulating hormone assay thyroid-stimulating hormone test.
Treponema pallidum hemagglutination assay (TPHA) a treponemal antigen serologic test for syphilis using tanned sheep red blood cells coated with antigen from the Nichol's strain of Treponema pallidum and treated patient serum; it is similar in sensitivity and specificity to the FTA-ABS test. This test is not useful for individuals who have had syphilis in the past.

ELISA

(ĕ-lē'să),

ELISA

(ĭ-lī′zə, -sə)
n.
A sensitive immunoassay that uses an enzyme linked to an antibody or antigen as a marker for the detection of a specific protein, especially an antigen or antibody. It is often used as a diagnostic test to determine exposure to a particular infectious agent, such as the AIDS virus, by identifying antibodies present in a blood sample.
Enzyme-Linked Immunosorbent Assay. An immunoenzymatic assay used to measure antigens and antibodies which approaches the sensitivity of RIA
Pros Lower cost, simpler equipment, faster ‘turn-around time’, and none of the problems inherent in handling radioactive substances

ELISA

Enzyme-linked immunosorbent assay Lab medicine A heterogeneous immunoenzymatic assay that approaches the sensitivity of RIA Pros Lower cost, simpler equipment, faster 'turn-around time', and none of the problems inherent in handling radioactive substances; ELISA may be used to measure any antigen and antibody. See Avidin-biotin method, EMIT, RIA, Sandwich method.

ELISA

Abbreviation for enzyme-linked immunosorbent assay.

ELISA

see ENZYME-LINKED IMMUNOSORBENT ASSAY.

ELISA

Enzyme-linked immunosorbent assay. This test has been used a screening test for AIDS for many years and has also been used to detect gonorrhea bacteria.
Mentioned in: Gonorrhea
References in periodicals archive ?
The specificity of our ELISA assay was further assessed by analyzing Gas6-depleted plasma.
Contrary to the TK REA assay, the ELISA assay can easily be standardized by calibration against AZTMP and use of a fixed conversion rate between IU/L and AZTMP formed in the assay, which is another major advantage of the new method.
Multiplex microarray-based ELISA assays have been reported to provide concordant results when compared with ELISA-based tests [3] with the added advantage offered by multiplexing reduced labor and provision of the complete autoantibody profile with a single test.
(PANG-OTCBB, NASDAQ) has signed an exclusive three year laboratory service agreement with Gamma-Dynacare Medical Laboratories to establish and perform the ELISA assays; and will perform additional clinical trials confirming the efficacy of the test.
This study was undertaken in order to develop a standardised protocol for a more sensitive and specific serological ELISA assay to detect the PGL-I antibody in patients and household contacts screened at Reference Centres.
The results of the study in cats showed that when both the urine dipstick and SSA test results were in the trace to 1+ range, the positive results are best confirmed with a more specific-specific ELISA assay. Detection of albumin in urine from cats should always be confirmed with this highest quality assay.
Serodiagnosis of leptospirosis by an IgM-specific ELISA assay is often used as an alternative to MAT in routine diagnostic laboratories (16).
Antibody-based ELISA assay was included in the current study to compare it with the other tests used.
The new BetaMark(tm) sAPPbeta ELISA assay, available only from Covance, provides researchers with a two-step process for obtaining reproducible, consistent, and ultra-sensitive detection of sAPPbeta in a variety of samples.
coli 0/76 (VT1 and VT2) Clostridium difficile 3/29 Toxins (10.3%) Wet Mount for ova/ 1/76 parasite (1.3%) * Giardia ELISA Assay 1/76 (1.3%) Adenovirus 2/76 (2.6%) Rotavirus 0/76 * The wet mount was positive for Giardia.