ELISA


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Related to ELISA: Western blot, ELISA test

assay

 [as´a]
determination of the purity of a substance or the amount of any particular constituent of a mixture.
biological assay bioassay; determination of the potency of a drug or other substance by comparing the effects it has on animals with those of a reference standard.
CH50 assay a test of total complement activity as the capacity of serum to lyse a standard preparation of sheep red blood cells coated with antisheep erythrocyte antibody. The reciprocal of the dilution of serum that lyses 50 per cent of the erythrocytes is the whole complement titer in CH50 units per milliliter of serum.
enzyme-linked immunosorbent assay (ELISA) any enzyme immunoassay using an enzyme-labeled immunoreactant (antigen or antibody) and an immunoadsorbent (antigen or antibody bound to a solid support). A variety of methods are used for measuring the unknown concentration, such as either competitive binding between the labeled reactant and unlabeled unknown or a sandwich technique in which the unknown antigen binds both the immunoadsorbent and labeled antibody. One of the uses of ELISA is to screen blood for antibody to the human immunodeficiency virus; a positive result indicates probable exposure to the virus and possibly that the virus is in the blood. Since false-positives can occur, a back-up test is used to confirm positive findings.
 ELISA: With antigen capture ELISA, the wells are coated with antibody to the virus. The sample containing virus (1) is added and, after washing several times, enzyme conjugated to an antibody to the virus is added (2). Finally, after a further cycle of washing, the enzyme's substrate (3) is added. A colored product is formed if the viral antigen is present in the sample (4). From Hart and Shears, 1997.
microhemagglutination assay–Treponema pallidum (MHA-TP) a Treponema pallidum hemagglutination assay using microtechniques; used in the detection of syphilis.
radioreceptor assay a radioligand assay in which a radiolabeled hormone is used to measure the concentration of specific cellular receptors for the hormone in tissue specimens, an example being radioassay of estrogen receptors in breast tissue.
thyroid-stimulating hormone assay thyroid-stimulating hormone test.
Treponema pallidum hemagglutination assay (TPHA) a treponemal antigen serologic test for syphilis using tanned sheep red blood cells coated with antigen from the Nichol's strain of Treponema pallidum and treated patient serum; it is similar in sensitivity and specificity to the FTA-ABS test. This test is not useful for individuals who have had syphilis in the past.

ELISA

(ĕ-lē'să),

ELISA

(ĭ-lī′zə, -sə)
n.
A sensitive immunoassay that uses an enzyme linked to an antibody or antigen as a marker for the detection of a specific protein, especially an antigen or antibody. It is often used as a diagnostic test to determine exposure to a particular infectious agent, such as the AIDS virus, by identifying antibodies present in a blood sample.
Enzyme-Linked Immunosorbent Assay. An immunoenzymatic assay used to measure antigens and antibodies which approaches the sensitivity of RIA
Pros Lower cost, simpler equipment, faster ‘turn-around time’, and none of the problems inherent in handling radioactive substances

ELISA

Enzyme-linked immunosorbent assay Lab medicine A heterogeneous immunoenzymatic assay that approaches the sensitivity of RIA Pros Lower cost, simpler equipment, faster 'turn-around time', and none of the problems inherent in handling radioactive substances; ELISA may be used to measure any antigen and antibody. See Avidin-biotin method, EMIT, RIA, Sandwich method.

ELISA

Abbreviation for enzyme-linked immunosorbent assay.

ELISA

see ENZYME-LINKED IMMUNOSORBENT ASSAY.

ELISA

Enzyme-linked immunosorbent assay. This test has been used a screening test for AIDS for many years and has also been used to detect gonorrhea bacteria.
Mentioned in: Gonorrhea
References in periodicals archive ?
Early generations of ELISA had a long incubation period that has been reduced in new generations.
Elisa is a telecommunications and ICT service company.
Despite this limitation, ELISA is a useful tool, says Ching-Hua Huang, an environmental engineer at the Georgia Institute of Technology.
As Elisa was happy with her base colour, Andy just added slices of colour through her hair in seven different shades of red, burgundy and plum.
ELISA detects antibody to anti-human leukocyte antigen (HLA) and can tell whether the patient is presensitized, or whether the transplant is sensitizing them, she said.
La utilizacion de la prueba de ELISA, como un sistema de chequeo de parvadas, es el metodo mas efectivo, ya que permite hacer un seguimiento de titulos y detectar anormalidades en la curva de anticuerpos, en comparacion a un estandar establecido por cada empresa.
The ELISA is used for initial screening because of both its high sensitivity and its practical advantages, Liska adds: "It's a lot easier to do many specimens on an ELISA.
We report for the first time development and performance characteristics of a novel noncompetitive ELISA for total IGFBP-1 that is unaffected by changes in its states of phosphorylation.
Wearing suits and solemn expressions, New York Mayor Rudolph Giuliani and the city's commissioner of the Administration of Children's Services recently held a press conference to announce the suspension, for 30 days without pay, of the caseworker and supervisor who had mishandled the Elisa Izquierdo case.
The test, an enzyme-linked immunosorbent assay (ELISA), looks for an antibody reaction to the trichina worm.
12 June 2019 - Finland-based telecommunications and digital services provider Elisa has agreed to acquire 100% of Sweden-based Polystar OSIX AB group shares, the company said.