Full-length mouse Egr3 gene (NM_018781.3) was amplified by PCR from the mouse ovary cDNA sample .
For live imaging of Egr3 expression in mouse oocytes, full-length and truncated Egr3 cRNAs were produced by in vitro transcription using mMessage mMachine T7 transcription kit (Ambion, Austin, TX, USA).
To visualize Egr3 localization by live imaging, Egr3-DsRed2 or truncated Egr3-DsRed2 cRNA (0.0034 [micro]M) was microinjected into GV oocytes.
Egr3 Selectively Regulates Late Phase of Arc Transcription in DG Granule Cells.
This interaction demonstrates that while Arc expression remains normal in [Egr3.sup.-/-] mice both during rest (i.e., in caged controls) and early after behavioral induction (i.e., in the 5' and 60' groups), the late phase of Arc expression (i.e., 240' and 480') is significantly reduced in mice lacking Egr3.
Expression of estrogen-responsive genes, After MCF-7 cells were exposed to a series of PFCs for 48 hr, the expression levels of two estrogen-responsive genes (EGR3 and TFF1) were analyzed by real-time PCR.
Transcription factor EGR3 is involved in the estrogen-signaling pathway in breast cancer cells.