The CpG dinucleotides we studied were located inside a MYC protooncogene (MYC), MYC-associated factor X (MAX), enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), SUZ12 polycomb repressive complex 2 subunit (SUZ12), and E2F transcription factor 6 (E2F6), domain sites that were determined by ChiP experiments (12).
Dnmt3b recruitment through E2F6 transcriptional repressor mediates germ-line gene silencing in murine somatic tissues.
 Nonstandard abbreviations: MetS, metabolic syndrome; LPL, lipoprotein lipase; VAT, visceral adipose tissue; HOMA-IR, homeostatic model assessment of insulin resistance; HDL, high-density lipoprotein; VLDL, very low-density lipoprotein; BMI, body mass index; ApoA1, apolipoprotein A1; ApoB, apolipoprotein B; HRP, horseradish peroxidase; MYC, MYC protooncogene; MAX, MYC-associated factor X; EZH2, enhancer of zeste 2 polycomb repressive complex2 subunit; SUZ12, SUZ12 polycomb repressive complex 2 subunit; E2F6, E2F transcription factor 6; PRC2, polycomb repressive complex 2.
Let-7 also inhibits cell proliferation by regulating transcriptional factors such as STAT3, E2F5, E2F6
, CBFB, PLAGL2, SOX9, GZF1, YAP1, GTF2I, and ARID3A [42, 43].
Using qRT-PCR, we checked the enrichment of these targets in the pool of miR-28-5p-captured targets (miR-28 pull out sample) and found that RAP1B, N4BP1, MPL, MAPK1, TEX-261, and E2F6 were enriched by more than 2-fold in the miR-28-5p pull out sample (Figure 3(a)).
To verify whether the enrichment of the selected targets in the miR-28-5p pull out sample was indicative of the miRNA regulatory function, we selected the most enriched one, that is, E2F6, and determined its expression after the miR-28-5p reexpression in DU-145 cells.
HPV E7 targets E2F6
, an important component of PRC2.
RESULTS: We have found that WPE-1 NB26 cells express less miR-205 and miR-31 than WPE-1 NA22 cells, and hence have higher levels of anti-apoptotic proteins like Bcl-w and E2F6
It has also been shown that miR-193a-3p decreased the abilities of proliferation by the expression inhibition of some TFs, including E2F6
, and other genes involved in the growth of several cancer types, for example, K-Ras, ERBB4, and cyclin D1 [3,8,27,28].
Phosphatidic acid phosphatase type 2A (PPAP2A), E2F6, and S1Pr2 genes were related to the enriched pathway.
In the small intestine, all genes (BRMS1, DSP, E2F6, NUMA1, and PPAP2A) except S1PR2 were directly connected with ubiquitin C to form a network concerned with cancer, cell to cell signaling and interaction and cellular growth and proliferation.
Intestine Ensembl Gene ID Gene Symbol Gene Description ENSSSCG00000002523 CDC42BP B CDC42 binding protein kinase beta (DMPK-like) [Source :HGNC Symbol;Acc:1738] ENSSSCG00000019556 7SK.46 7SK RNA [Source:RFAM;Acc:RF00100] ENSSSCG00000012939 BRMS1 breast cancer metastasis suppressor 1 [Source:HGNC Symbol;Acc:17262] ENSSSCG00000013658 S1PR2 sphingosine-1-phosphate receptor 2 [Source:HGNC Symbol;Acc:3169] ENSSSCG00000026161 E2F6
E2F transcription factor 6 [Source:HGNC Symbol;Acc:3120] ENSSSCG00000001025 DSP desmoplakin [Source:HGNC Symbol;Acc:3052] ENSSSCG00000014802 NUMA1 nuclear mitotic apparatus protein 1 [Source:HGNC Symbol;Acc:8059] ENSSSCG00000016912 PPAP2A phosphatidic acid phosphatase type 2A [Source:HGNC Symbol;Acc:9228] ENSSSCG00000030936 CH242-154K8.2 b.