DNA sequencing

(redirected from Dye terminator sequencing)
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DNA sequencing

n.
The determination of the sequence of nucleotides in a sample of DNA.
The American Heritage® Medical Dictionary Copyright © 2007, 2004 by Houghton Mifflin Company. Published by Houghton Mifflin Company. All rights reserved.

base-sequence analysis

A generally automated method for determining the order of nucleotide bases in a nucleic acid (DNA or RNA).
Segen's Medical Dictionary. © 2012 Farlex, Inc. All rights reserved.

DNA sequencing

The determination of the sequence of base pairs in a length of DNA.
Collins Dictionary of Medicine © Robert M. Youngson 2004, 2005

DNA sequencing

determination of the order of NUCLEOTIDES in a DNA molecule or part thereof. A number of techniques is available to determine a DNA sequence, for example the chain-terminator DNA sequencing procedure (see DIDEOXYRIBONUCLEOTIDE). Generally such techniques rely on the power of polyacrylamide gels to resolve NUCLEIC ACID molecules that differ in length by only a single nucleotide. Ladders of DNA fragments are formed on the gels following ELECTROPHORESIS. Both manual and automated methods of sequencing are available.
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005
References in periodicals archive ?
Gel-purified PCR products were quantitated, and the partial nucleotide sequences of amplicons with a minimum of 60 ng DNA/[micro]L were determined by using Big Dye terminator sequencing ready reaction kits with AmpliTaq DNA polymerase FS (Applied Biosystems, Foster City, CA).
Sequencing reactions were conducted with the Big Dye Terminator Sequencing Ready Reaction reagent set (Perkin-Elmer) according to the manufacturer's instructions.