Cells were cultured in Dulbecco's medium
(Cultilab[R]) with 5% FCS, sodium bicarbonate, and 40 mg/ml gentamicin.
GIBCO[R] Iscove's Modified Dulbecco's Medium
(IMDM) and GIBCO[R] Phosphate Buffered Saline (PBS) were purchased from Invitrogen[TM] Ltd, United Kingdom.
Hematopoietic Growth Progenitor Medium (HGPM) and Iscove's Modified Dulbecco's Medium
(IMDM) were procured from Lonza.
8 mE), the sample may be diluted 1:1 with Dulbecco's medium
(Gibco, Grand Island, NY).
1 bloodstream-form of trypomastigotes were maintained in Isocove's modified Dulbecco's medium
(IMDM) with various supplements containing 10% heat-inactivated fetal bovine serum (FBS) at 37 [degrees]C, under 5% [CO.