Anticoagulant-treated blood was diluted with an equal volume of Iscove's modified Dulbecco's medium
HUVE-12 cells were obtained from the China Center for Type Culture Collection (Wuhan, China) and maintained in Iscove's modified Dulbecco's medium
supplemented with 10% fetal bovine serum at 37[degrees]C in a humidified incubator containing 5% CO[sub]2.
The Vero cells were propagated as monolayers in complete Dulbecco's medium
containing 10% fetal bovine serum and supplemented with penicillin and streptomycin (10 unit/ml Biowest).
Then the cells were collected by centrifugation at 1000 rpm for 5 min and re-suspended in Iscove's Modified Dulbecco's Medium with 10% FBS and 10 ng/ml M-CSF.
In brief BMDM cells were cul-tured in Iscove's Modified Dulbecco's Medium (IM-DM) containing 10% FBS 100 ng/ml LPS and 50 ng/ ml IFN- for 48 h with or without exosomes treatment.
Cells were cultured in Dulbecco's medium
(Cultilab[R]) with 5% FCS, sodium bicarbonate, and 40 mg/ml gentamicin.
GIBCO[R] Iscove's Modified Dulbecco's Medium
(IMDM) and GIBCO[R] Phosphate Buffered Saline (PBS) were purchased from Invitrogen[TM] Ltd, United Kingdom.
Hematopoietic Growth Progenitor Medium (HGPM) and Iscove's Modified Dulbecco's Medium
(IMDM) were procured from Lonza.
8 mE), the sample may be diluted 1:1 with Dulbecco's medium
(Gibco, Grand Island, NY).
The hybridoma cells were resuspended in 90 mL of Iscove's modified Dulbecco's medium
(Sigma Chemical Company) (IMDM) containing 200 [[micro]liter] [mL.
1 bloodstream-form of trypomastigotes were maintained in Isocove's modified Dulbecco's medium
(IMDM) with various supplements containing 10% heat-inactivated fetal bovine serum (FBS) at 37 [degrees]C, under 5% [CO.