The HUVECs were seeded in 24-well plates at a density of 6000 cells/[cm.sup.2] and incubated overnight in Dulbecco
's modified Eagle's medium.
Au cours du premier atelier ayant trait aux politiques publiques en matiere de numerique dans l'enseignement scolaire dirige par Monsieur Philippe Dulbecco
seront examines, dans le cas de la France, le numerique en tant que levier de transformation des ecosystemes scolaires et, dans le cas du Maroc, la politique publique en matiere de numerique dans le scolaire.
1: Concentration of phenol red in different media formulations from different sources (in [micro]M) Source DMEM MEM DMEM/F-12 RPMI 1640 IMDM Thermo-Fisher[R] 39.85 26.57 21.57 13.28 39.85 Lonza[R] 40.76 26.57 13.55 40.76 ATCC[R] 42.33 22.85 14.11 42.33 Sigma-Aldrich[R] 44.87 31.04 24.35 14.96 45.15 DMEM, Dulbecco
's Modified Eagle's Medium; MEM, Minimum Essential Medium; DMEM/F-12, DMEM/Ham's F12; RPMI, Roswell Park Memorial Institute; IMDM, Iscove's Modified Dulbecco
All cell lines were cultured at 37AdegC, 5% CO2 with Dulbecco
's modified Eagle's medium (DMEM), supplemented with 10% heat-inactivated FBS and 1% of 100 times diluted 10,000 IU/ml of penicillin-10,000 ug/ml of streptomycin.
To overcome this, we washed the adipose tissue with HBSS or Dulbecco
's phosphate-buffered saline (DPBS).
The MAC-T cells were cultured in three types of Dulbecco
's modified Eagle's medium (DMEM; Hyclone, Waltham, MA, USA) based on concentration of glucose (no-glucose: 0 g/L, low-glucose: 1 g/L, and high-glucose: 4.5 g/L), containing 10% fetal bovine serum (FBS; Hyclone), 100 IU/mL penicillin, and 100 pg/mL streptomycin (Sigma, St.
Cell lines of human colon adenocarcinoma Caco-2 (Sigma-Aldrich) were used, they were maintained in Dulbecco
Modified Eagles Medium, DMEM (Sigma-Aldrich) supplemented with 20 % fetal bovine serum (FBS) and 1 % penicillin / Streptomycin.
The following culture media were used: Dulbecco
's modified Eagle's medium (DMEM) (Gibco Invitrogen Corporation), gentamicin (Gibco) from Novafarma; streptomycin/penicillin (Gibco) from Ache, supplemented with 10% fetal bovine serum (FBS) (Gibco), mouse fibroblast NIH-3T3 cells by American Type Culture Collection (ATCC CRL-1658), methanol > 99% purity (Telemetrics Inc.), amoxicillin/clavulanate (GSK), xylazine and ketamine (Vet Brands), mononylon 3.0 (Point Suture), tramadol (generic Legrand), formaldehyde (Coremal), and hematoxylin-eosin (Merck).
Before cell seeding, all CPC and CPC-WF disks were immersed in Dulbecco
's modified Eagle's medium (DMEM) for 3 days, with medium replaced daily.
The solvents for the suspensions, chosen for their biological relevance, were as follows: ultra-pure water, Fusayama and Mayer artificial saliva (AS) prepared according to  (pH = 5.3-5.9), 0.1 M phosphate buffer saline solution (PBS, tablets, Sigma Aldrich) (pH = 7.47), and Dulbecco
's Modified Eagle Medium for cell cultures (DMEM 1x with Glutamax-I, 1g/L D-Glucose, pyruvate, Gibco, Life Technologies) used as received (DMEM 1: 1, pH = 7.47) or diluted 100 times in ultra-pure water (DMEM 1: 100, pH = 7.15).
The culture medium Dulbecco
's modified Eagle's medium (DMEM) used in all the experiments was purchased from Lonza.