Dragendorff

Dra·gen·dorff

(drag'ĕn-dōrf),
Georg J.N., German physician and pharmaceutical chemist, 1836-1898. See: Dragendorff test, Dragendorff reagent.
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Dragendorff's and Mayer's reagent test was performed for detection of alkaloids.
Dragendorff's test: Samples were spotted on TLC plate and Dragendorff's reagent was applied.
Microscopic analysis/Staining: The Prussian blue method (Price & Butler, 1977) for phenolics and the Dragendorff assay for alkaloids (Furr & Mahlberg, 1981) were employed in histochemical tests.
Long Short (365 nm) (254 nm) 1 Blank Straw yellow Brown Straw yellow 2 Water Cinnamon Cinnamon Light brown brown brown 3 Mayer's Grayish Dark brown Pastel yellow orange 4 Hager's Cream Grayish Grayish brown orange 5 Phloroglucinol Brownish Violet Dark brown yellow brown 6 Barfoed's Dark brown Grayish Violet brown reagent brown 7 Dragendorff's Brownish Brown Light brown reagent orange UV: Ultraviolet Table 2.
Dragendorff's reagent was added to 1mL of infusion extract for alkaloid determination.
Dragendorff's test: 1 mL of extract + 1 mL of Mayer's reagent.
Se adicionaron 10 mL de CHCl3, recuperando la fase cloroformica, este proceso se repitio hasta extraer todos los alcaloides desde la fase acuosa hacia el extracto cloroformico, para esto se realizaron evaluaciones en la fase cloroformica mediante cromatografia en capa fina y con el reactivo de Dragendorff. El contenido de alcaloides fue concentrado, eliminando el solvente mediante un rotavapor (HiTech, RE-52), con una temperatura de 40 [degrees]C y presion controlada.
longa [5,6] Chemical Method Result substance tested Alkaloid Mayer's test + Dragendorff's test + Hagers test + Wagner's test + Tannins present Ferric chloride test + Gelatin test + Lead acetate test + Flavonoids Ferric chloride test + Magnesium hydrochloride test + Sodium hydroxide test + Mineral acid test + Carbohydrates Molisch's test + Benedict's test + Fehling's test + Barfoed's test - Phytosterols Liebermann-burchard test + Proteins and amino Millions test - acids Biuret test - Ninhydrin test - Lactones Acetic anhydride test + Alkaline nitroprusside test + Triterpenes Salkowski test + Liebermann-storch mora sky + test Hirshhorn test + Anthraquinone Borntrager's test + glycosides C.
Potential classes of potential antibacterial phytochemicals such as alkaloids (Dragendorff's and Mayer's tests), terpenoids: sterols (Salkowski's test), saponins (foam test) and triterpenes (Liebermann-Burchard test), and phenolics: anthraquinones (Borntrager's test), flavonoids (aluminum chloride test), polyphenols (ferric chloride test), and tannins (gelatin test) (Table 2) were investigated according to the described phytochemical methods [19, 20].
These tests included 2,4-dinitrophenylhydrazine (carbonyl group), anthrone (carbohydrates), Baljet (sesquiterpene lactones), sodium bicarbonate (carboxyl group), ferric chloride (tannins), Dragendorff (alkaloids), Liebermann-Burchard (sterols and triterpenes), potassium permanganate (double bonds), Shinoda (flavonoids), sodium hydroxide (coumarins), Borntrager (quinones), and sodium bicarbonate (saponins).