Drabkin reagent

Drab·kin re·a·gent

(drab'kin),
a solution used in the cyanmethemoglobin method of measuring hemoglobin. It consists of sodium bicarbonate, potassium cyanide, and potassium ferricyanide.
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Blood samples were mixed at 1:10, 1:15, or 1:20 ratios (vol/vol) with Drabkin reagent (Ricca Chemical Co.
We performed this paper-based Hb assay by first mixing a sample of whole blood with Drabkin reagent and then depositing a 20-[micro]L droplet of the mixture onto the [micro]PAD and letting the blood stain develop (Fig.
We then mixed a small volume of each calibration sample with Drabkin reagent at 1:10, 1:15, and 1:20 ratios (vol/vol), waited 10 min, and placed a 20-[micro]L droplet of each mixture onto paper.
For the GPX assay, hemolysates (50 [micro]L) were diluted with 1 mL of Ransel diluting agent (Ransel reagent; Randox) and incubated for 5 min, followed by the addition of 1 mL of the Drabkin reagent and mixing (total dilution of the hemolysates before assay, 1:82).