Dot Blot


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A rapid—‘quick and dirty’—hybridization technique for semiquantifying a specific RNA and DNA fragment in a specimen without performing a more time-consuming Northern and Southern blot
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An innovative character of the dot blot, which was used in the present study, is in the fact that in the SSHL diagnostics, in addition to free Hsp70, the Hsp70 bound to circulating immune complexes (CIC) is also searched for.
This result confirms previous studies [20,21] that used a dot blot or indirect ELISA techniques based on extraction of rough soluble antigen from [RB.sub.51] vaccinal strain.
In the present study, we studied the distribution patterns of 5hmC, 5fC, and 5caC in the postnatal mouse brain, cultured adult neural stem cells (aNSCs) with immunostaining and dot blot, and compared their global levels.
HAR is a common high-altitude illness, which is clinically characterised by increased dilatation and tortuosity of retinal vasculature along with diffuse dot blot and flame shaped haemorrhages, usually involving mid-periphery.
The homogenate was centrifuged at 12,000 g for 20 min at 4 C and the resulting supernatant was used for dot blot analysis.
These values fall under the Kappa interpretation of almost perfect agreement (0.81-0.99) and substantial agreement (0.61-0.80) for the ELISA and the dot blot, respectively, where Kappa index of 1 is perfect agreement [29].
The synaptosomes were further treated to isolate dendritic rafts and PSDs, and dendritic rafts and PSDs were analyzed by Western blot or dot blot.
The content of 3-NT positive proteins in lung tissue, determined by dot blot analysis, was significantly increased in the PAH group and reduced by PETN (Figure 4(d)).
Furthermore, detection of TK1 protein was carried out in 96 patients using a chemiluminescence dot blot assay.
abortus S19 and Brucella abortus S19[DELTA]per S19 was checked using dot blot technique.
The system can detect up to 22 targets per sample using reverse dot blot technology and can detect genus, species, strains and virulence factors in a single assay.